APPD (PLEKHF1) (NM_024310) Human Mass Spec Standard

Specifications

Product Data
Description	PLEKHF1 MS Standard C13 and N15-labeled recombinant protein (NP_077286)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC201282
Predicted MW	31.2 kDa
Protein Sequence	Sequence Link (showhide) >RC201282 protein sequence Red=Cloning site Green=Tags(s) MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLN KRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRP PSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRE LAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_077286
RefSeq Size	1774
RefSeq ORF	837
Synonyms	APPD; LAPF; PHAFIN1; ZFYVE15
Locus ID	79156
Cytogenetics	19q12
Summary	May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.[UniProtKB/Swiss-Prot Function]

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