TFIIF (GTF2F2) (NM_004128) Human Mass Spec Standard

Specifications

Product Data
Description	GTF2F2 MS Standard C13 and N15-labeled recombinant protein (NP_004119)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200361
Predicted MW	28.4 kDa
Protein Sequence	Sequence Link (showhide) >RC200361 protein sequence Red=Cloning site Green=Tags(s) MAERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGG KPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVR LSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPV VYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEKSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_004119
RefSeq Size	1469
RefSeq ORF	747
Synonyms	BTF4; RAP30; TF2F2; TFIIF
Locus ID	2963
Cytogenetics	13q14.12-q14.13
Summary	TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.[UniProtKB/Swiss-Prot Function]
Protein Families	Transcription Factors
Protein Pathways	Basal transcription factors

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