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Pik3ca Mouse Gene Knockout Kit (CRISPR)
CAT＃: KN313294RB
Pik3ca - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
CNY 12,260.00
Product images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Pik3ca |
| Locus ID | 18706 |
| Kit Components |
KN313294G1, Pik3ca gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCACCCGAAGATGGTCGTGG KN313294G2, Pik3ca gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATTCCACTAGGATTCGTGG KN313294RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACCTCAA ATGATAGTAA CAGTGGCAGA AGCCATCTTC GTGTGTGCCA GGCAAGTGCT CACTACTCCA TCCCATCCCT AACCTGTTGG CTGCTGCTAC TTGAGACATG CTTGCTAAGT GTTCCAGGCA AGCCTTGAAC CTGCAAATAA ATCCCCCTGC CTCAGCCAAA GGAGCTGAGG TTGCAGGCCT GCACCACCAA GCCTGGGGAG ATGGTCATTT CTGGTTGAGT TACAGCTTGC TTTTTCATTC TTCCCATTGC TTGTTTTTGT GAGACCCAGC TTAGAAAACC TATTCAATAT TTTTAAATTT TCATTAAATC TAATTAAATG CAGGTTATTT TTCTACTGTC TTCTGTAATA AAATCTGTGT AGCCTAGTTT AGAGCAACCA TCTAGATTAA AACACATAAG TTATTACTGC TCTGATTAGT CAGACAACAA GTTGTGACCA TTTGCACTTC TCAGTGCTGT CGTCAGTGTG TGATCAGCAA TTGCTTCTCT CTAACAGGTG GTTTTCTTCT TTTGTTCAGG GCACTGTTTG GAGACAGTCG TGATTCTGAC TCCATAAGGC GGTTTTCTAT GTAAAGTTTG CAGAGGGTCA GAGCAAATGA TAGTGACTTT AGAATGCCTC CGTGAGGCCA CACTCGTCAC CATCAAACAT GAACTGTTCA GAGAGGCCAG GAAATACCCT CTCCATCAGC TTCTGCAAGA CGAAACTTCT TACATTTTCG TAAGTGTCAC CCAAGAAGCA GAAAGGGAAG AATTTTTTGA TGAAACAAGA CGACTTTGTG ACCTTCGGCT TTTTCAACCC TTTTTAAAAG TTATTGAACC AGTAGGCAAC CGTGAAGAAA AGATCCTCAA TCGAGAAATT GGTATGATAT AATTTTCAGC TAATTGACAC TGTCATTGCT AAACTGCAAA TAACTATGAT GCCTCCTCTT TATTTTATTC TTAAGATACT TTGAATTATT ACCTTTGTAA TCTTTGAGTA GATTTCTATT TAAAAATATA AAGTAGGGTT TTTGTTTTTA TGGTTTTTTA CTACTGAGCT ATAGAACTTC GTAACGCTTT GATATCCTGT AGGGTTTTAC TTAAGGGAAA CAATTAGTAG GAGGACTTAG ATTTCTTATT AGCCAAGTTT TATTACTCAG AAAAAGTTGA TAAAGGTCCT ATCATAATAT TAATGGATAT ATGGACAATG CATATTCACT CTCGCCGGTT GGACTTAGAT CAGAAGGGAT CTTGCTGCCG CCCGAAAGAG GAAGGGCTGG AAGAGGAAGG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_008839 |
| Synonyms | 6330412C24Rik; caPI3K; p110; p110alpha |
| Summary | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Plays a role in the positive regulation of phagocytosis and pinocytosis (PubMed:19604150).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
| 基因表达相关资源 |
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| KN313294 | Pik3ca - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
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| KN313294BN | Pik3ca - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
|
| KN313294LP | Pik3ca - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
|
| KN513294 | Pik3ca - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. |
CNY 8,680.00 |
|
| GA203215 | Pik3ca CRISPRa kit - CRISPR gene activation of mouse phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha |
CNY 12,255.00 |
