Nlrp12 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN311043RB

Nlrp12 - mouse gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

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CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Goat Anti-Nalp12 / Nlrp12 (rodent) Antibody
    • 100 ug

CNY 5,371.00


Nlrp12 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 12 (Nlrp12)
    • 10 ug

CNY 11,008.00

Specifications

Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD
Symbol Nlrp12
Locus ID 378425
Kit Components

KN311043G1, Nlrp12 gRNA vector 1 in pCas-Guide CRISPR vector

KN311043G2, Nlrp12 gRNA vector 2 in pCas-Guide CRISPR vector

KN311043RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001033431
Synonyms Nalp12; PYPAF7
Summary Plays an essential role as an potent mitigator of inflammation (PubMed:26521018, PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:30559449). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the DDX58-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked DDX58 degradation (By similarity). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (PubMed:30212649).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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