Lats2 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	Lats2
Locus ID	50523
Kit Components	KN309148G1, Lats2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GATTGCAAGAGATTCGAGAG KN309148G2, Lats2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATCCAAGGCTTCCACCCAG KN309148RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet CTCAGGTGGG TGAATATGTT AGGTAAAGCC TCAGGCTTAG GGACAGTGGT GTGAATCAGG AATTGGTTGA TATTAGGGAC AAGCCAGAAA TTTACTGGGT ATGTTTAACC CTAAATCCCC AAGCTGTGGT TTCCAGAATT ACATGATAGT GAGTTGTTGA GACGCCCGCC GTTGCCCTGG GTGGTGGTTG TGGAGACACT TGGCTGTAGG CTTCCTGTTA AAGGGACCTT CCTGGGACAG CTCCAGAGCC AAGTAGCCCA TGACACTTCT GAAAGCCATA TACTTTTTCT TTACTCTCTA GCTGGAATAT TTCCAAAATC ACCATGCGTT TCTGGAAAGT AGCTTTAACA TTATGTGAAA TCCAAAAATA ACCACTTTTG TCCCATTTTC TTTTTCAGAG AGTGATGGTC TTCAAAATGA AAACTCTGGA AAATTTTAGG TTCTCTTTAG GAACTACAAA AATTGAAGGA CAGCAATTTT TTGAAAGGAA GTTGTTCTGA AAGCATGTTT ACAATTCACT GACACTGTTG ACTGTTCTCT TTAAAATAAT AAGACGCTTT GAGAAGATTG TATTTATGGT AAAAGGAAAC TGGACTAACA GACCAAACAG TGACACTTCC CTGGATGCCA AAGTCCTGGG GAGCAAAGAT GCCTCCAGGC AGCAGCAAAT GAGAGCCACC CCGAAGTTTG GACCTTATCA AAAAGCTCTC AGGGAAATCC GATATTCCCT CCTGCCTTTT GCCAACGAGT CAGGCACTTC GGCAGCTGCA GAGGTGAACC GGCAGATGCT TCAGGAGTTG GTGAATGCGG GATGTGACCA GGTGGGTCCA GGTGCCAGCG CCCTTCAGCA GCTCTGGGCA AGCTCTGAAC TCTTCTCACA CTGCCCTTTT CTGTATCCCT TAGCCAGTGG CTGCCTTCTC TCCAAGGGTC TTAGGCACTT TTCCTTCTTG AAATTGTTCC TTGCTGCTTT TTCATTCATT CTGCTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTC TAGAACCCAG GGTCCTGTGC ATTTGAAGCA CACGCTTCTC TGCTCAGCTG TGCCTGCTTC CAGTTCTATT CTTAGCGCTC TCCTTGGGAC CTGCTTGGGA TTATTCCGAG CTTCTGTGGA CAAGAGACTG GCTTCTCTTA CCTTGTTTTT GGTTTGTATT TTCATTTCCT TCCTGAACGA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_015771, NM_153382
Synonyms	4932411G09Rik; AV277261; AW228608
Summary	Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.