Klf15 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Klf15
Locus ID	66277
Kit Components	KN308828G1, Klf15 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACGAGAAGGTCTCGTCCAC KN308828G2, Klf15 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATGTGGTATGGCTGCCGGC KN308828-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CAGCCCAGAC AGATGTGTGT CCCATCCCTT CCAGTCCTGG GGACACCATG CTCACATCCA CAGATGTTCA TTTTTGAACA TCTAAAGTCA ACATCGAACA AGCAGCCTGT TTTTATTTGC TTAATCTGGA AACCCTACAG AAGCCCCAAT ACCTTGAGCT CAGAATGCAA ATGGCCTTTT CCTTTCTCAC CCCCTCTCTG ACTCAAGGTA GGAAGCAGAG TCCAGGGGCT GGCGACTTTC AGGGCCTCCC CTCTCTGGCT CCCTCTCTCC TCGTGGCCTC AGCTGTTCGC GGACAAGAGC GTTCCTTGTC TTATTCTTTT CCAGGATCAT TCTGGAAAAT GTTTGAAACC AGAAGTGCCC TGGATTGTGC AACGGGCCTG GAGAGGGAGG GCTCTTTTGC AAATAGGGTG ATGCAACTTT TGAAAACATA CTCGCCGGCC CCATTCTCCC TTGGGATGGA AGCAGGATCT GGACATTTGG CCACAGGAGG CCTTGGGCAG GCAGCCGAGG GTGAGGGCTG CAAGGCACTG GTAAGCAGTC CTTCTAACCT GGCCTCCCTT GTTCCCTCTC TGCAGTCACC ACAGGCTCGG CCAGGCCAGC CCCCTGCTCT TGTGCCAGCC CTGACTCGCA AGCCTTCTGT TCCTGCTACA GTGCGGGTCC AGGCCCTGAG GCCCAGGGCA GCATCTTGGA TTTCCTCCTG TCCCGGGCCA CACTGGGCAG TGGTGGTGGC AGTGGAGGTA TTGGAGATAG CAGTGGCCCT GTGACCTGGG GATCATGGAG GAGAGCCTCT GTGCCTGTGA AGGAGGAACA TTTCTGCTTC CCTGAATTTC TGTCAGGGGA CACTGATGAC GTCTCCAGGC CCTTCCAGCC TACCCTGGAG GAGATTGAAG AATTCCTGGA AGAGAACATG GAGGCTGAGG TCAAGGAGGC CCCAGAGAAC GGTAGCAGGG ACCTGGAGAC CTGTAGCCAG CTCTCAGCTG GGTCACACCG GAGCCACCTT CATCCAGAGT CTGCTGGGAG AGAGCGCTGT ACCCCACCAC CAGGTGGCAC GAGTGGGGGT GGTGCCCAAA GTGCAGGTGA GGGGCCAGCA CATGATGGCC CCGTGCCGGT GCTACTGCAG ATCCAGCCTG TTGCTGTGAA GCAGGAGGCA GGTACAGGGC CAGCCTCCCC AGGGCAGGCC CCAGAGAGCG TCAAGGTCGC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_023184, NM_001355668
Synonyms	1810013I09Rik; AV048136; AW494632; CKLF; KKLF
Summary	Transcriptional regulator that binds to the GA element of the CLCNKA promoter (By similarity). Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. Is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A, GATA4 and MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation (By similarity). Involved in podocyte differentiation.[UniProtKB/Swiss-Prot Function]

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