Klf15 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN308828

Klf15 - mouse gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

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CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (2)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15)
    • 10 ug

CNY 3,656.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol Klf15
Locus ID 66277
Kit Components

KN308828G1, Klf15 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACGAGAAGGTCTCGTCCAC

KN308828G2, Klf15 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATGTGGTATGGCTGCCGGC

KN308828-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

CAGCCCAGAC AGATGTGTGT CCCATCCCTT CCAGTCCTGG GGACACCATG CTCACATCCA CAGATGTTCA TTTTTGAACA TCTAAAGTCA ACATCGAACA AGCAGCCTGT TTTTATTTGC TTAATCTGGA AACCCTACAG AAGCCCCAAT ACCTTGAGCT CAGAATGCAA ATGGCCTTTT CCTTTCTCAC CCCCTCTCTG ACTCAAGGTA GGAAGCAGAG TCCAGGGGCT GGCGACTTTC AGGGCCTCCC CTCTCTGGCT CCCTCTCTCC TCGTGGCCTC AGCTGTTCGC GGACAAGAGC GTTCCTTGTC TTATTCTTTT CCAGGATCAT TCTGGAAAAT GTTTGAAACC AGAAGTGCCC TGGATTGTGC AACGGGCCTG GAGAGGGAGG GCTCTTTTGC AAATAGGGTG ATGCAACTTT TGAAAACATA CTCGCCGGCC CCATTCTCCC TTGGGATGGA AGCAGGATCT GGACATTTGG CCACAGGAGG CCTTGGGCAG GCAGCCGAGG GTGAGGGCTG CAAGGCACTG GTAAGCAGTC CTTCTAACCT GGCCTCCCTT GTTCCCTCTC TGCAGTCACC ACAGGCTCGG CCAGGCCAGC CCCCTGCTCT TGTGCCAGCC CTGACTCGCA AGCCTTCTGT TCCTGCTACA GTGCGGGTCC AGGCCCTGAG GCCCAGGGCA GCATCTTGGA TTTCCTCCTG TCCCGGGCCA CACTGGGCAG TGGTGGTGGC AGTGGAGGTA TTGGAGATAG CAGTGGCCCT GTGACCTGGG GATCATGGAG GAGAGCCTCT GTGCCTGTGA AGGAGGAACA TTTCTGCTTC CCTGAATTTC TGTCAGGGGA CACTGATGAC GTCTCCAGGC CCTTCCAGCC TACCCTGGAG GAGATTGAAG AATTCCTGGA AGAGAACATG GAGGCTGAGG TCAAGGAGGC CCCAGAGAAC GGTAGCAGGG ACCTGGAGAC CTGTAGCCAG CTCTCAGCTG GGTCACACCG GAGCCACCTT CATCCAGAGT CTGCTGGGAG AGAGCGCTGT ACCCCACCAC CAGGTGGCAC GAGTGGGGGT GGTGCCCAAA GTGCAGGTGA GGGGCCAGCA CATGATGGCC CCGTGCCGGT GCTACTGCAG ATCCAGCCTG TTGCTGTGAA GCAGGAGGCA GGTACAGGGC CAGCCTCCCC AGGGCAGGCC CCAGAGAGCG TCAAGGTCGC

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_023184, NM_001355668
Synonyms 1810013I09Rik; AV048136; AW494632; CKLF; KKLF
Summary Transcriptional regulator that binds to the GA element of the CLCNKA promoter (By similarity). Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. Is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A, GATA4 and MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation (By similarity). Involved in podocyte differentiation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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