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Ido1 Mouse Gene Knockout Kit (CRISPR)
CAT＃: KN308091RB
Ido1 - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
CNY 12,260.00
Product images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | Ido1 |
| Locus ID | 15930 |
| Kit Components |
KN308091G1, Ido1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CATCTATGTGGTGGTCTTCA KN308091G2, Ido1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTTTGCTCTACCACATCCAC KN308091RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACTGTCC CAGGTTGTGT TTCAGAGCTG GAGAGACAAA ACACTGACAT TTAAAACAGG ACATAAGTTC CCTCGTGTGA AAGCCAATAA CGGACAGCTA TTGCTAGTTC GGCTGTCTTT ATTTTAAACC CAAACTCTGA ACGTTTTCTA AAGCAAAATG CATTCTCCTG GTCACCTGTC CTCATGGTTC TTGTCATGTG AGTTGGGCAC TTTGTGGCTT GAACACCCAA TCCTCCTGCT GGGGTAACAG GTGGCCACCC AAACCACTGA TGGGGTGGTT TCCAGGGCCG AGGCCTGAGT GTGCACTCGG TCACCATAAT TGGCACTGGG AAGCTGAGGC CATCAGAGCC AACAGTTGAT GTCCTTATGG GATAAATCTG ATAAGAAACA CAAATGTTTA GTTTCTTCAT ATGACGAAGA GAGATCCTTT GTGGTTTGGT TGTTTATCAA ATTGCAAATA CATAAAACAG AAAATGAAAC TGTCCCAGGT GGAAGCCACT TCTCCCCGAG TATTACGTGT CAGCTGTGGG GTTTGGCTAA ACCCTATAAG AGATGCTGGG ACATTCCTTC AGTGGCCAAG TGGGGGGTCA GTGGAGTAGA CAGCAACAGA TGAATTTCTA AGTAAGCAGC TCTGTCTCAG GTTCTCTTAA GCGAATGAGT GATGTCAAAT TCAGAGCCCA CTTACAAAAA GGGAGCCTGG CTGCTCTTCA GTGTTTCTTC TTCTTCTTCT TCCTCAAAAT CCAGCCCTTA CTTTCTTAAC CTGGAAATGT GAAGTGGTGA GGGAAGGGGC ACATGAGAAG TCTTTTTAGT TTCGTGAAGG AAACGGGCTC ATAGAAGGGA AGCCAGTTTG TCACAATGAC TTTTCCAGCA CGGTGTCCTG AAACAGCAAC ATTCAAATGC CCAGAGCCAT AGAAACATTT CTCTTCTGAA CCTTATCAAA TGGATCGATC TGTGCTCCTC ACTGATTTCA GTATATGAGA TGATAGATCA AGCAACTTTT GTTTCATACT AATAATGCTT GTGGTTTGCC AAGCATAATC CAAAGGAAGG TATGAACAAC CTTGATTGTG CTTTTGTGCA AGTTTTGGAA TCCAAGTGCG GTGCATGTGG GGGAAATGCC TCGTGTCTCA ACCAGGTACA GTGGGAAACA ACTATAGTTC CATCACCCAG GAGTCAGAGG CAGGGGCATA GCCTCAAATT CAAGGTCACT CTCGCCGGTT GGACTTTAGA TCAGAAGGGA TCTTGCTGCC GCCCGAAAGA GGAAGGGCTG GAAGAGGAAG GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATCGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001293690, NM_008324 |
| Synonyms | Ido; Indo |
| Summary | Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity (PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection (Ref. 3).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
| 基因表达相关资源 |
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| KN308091 | Ido1 - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
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| KN308091BN | Ido1 - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
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| KN308091LP | Ido1 - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
|
| KN508091 | Ido1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. |
CNY 8,680.00 |
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| GA202058 | Ido1 CRISPRa kit - CRISPR gene activation of mouse indoleamine 2,3-dioxygenase 1 |
CNY 12,255.00 |
