Hgd Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA	mBFP-Neo
Symbol	Hgd
Locus ID	15233
Kit Components	KN307689G1, Hgd gRNA vector 1 in pCas-Guide CRISPR vector KN307689G2, Hgd gRNA vector 2 in pCas-Guide CRISPR vector KN307689BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. Homologous arm and mBFP-Neo sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet CATGAGGCTG TCAGCAGTTC ATGATCTGGG AGCTGAGCTC ATGCACAGGA GACAGTGGCA GAGAGCATCC AGTCCTTGTG CCCTTCTTCG GGCTGCTTGC ACCAGGTCAA AGCACATTGC AGGTTTTTTT CCATGGGCAC TGGATACTGG CCCTGCACCT TCCTGACCTG AGTCAGGACT AAGAGAGAAT TCTCTGTTAC ATCTGGCAGG TGCTAACTCT TAGATAGCTT TACATCCTCT GCCTTTGGGG AAAAAAAGAC TTCCTTAAAG TGGAACAAAT AATTGGTCAA TGTTTATATC AGGTGAAAGA GACCAGTTCC TGGGGAGGTG GAGGGAGCTC TCAGAAATGA CCTGGGGTTA AAAAAAAGAA AAAAAAAAAA GGTTGTGCAA GAATTTCGAT GTAAGCACAT TGGACTTTCT AACTAGTTTC TAACTTGTGT GTCAGTTAAG CAACTCTGCC CTGAGTGACC TCCCGACCAA GGAAACACAG AGAACTGGTA AAAATCAACA ACATCCGTCC AGAGAGGAGT TTTAAAGAGA CACGGGAACA TTGGTACCTC ACAAACTCCT TGGCCTGGGT CTCCCTCCAG CAGAAGGGAA CAACACTAGA AAAAGAAAGG ACAGAAAAGA AATGGGGAGA AAGGAAGGAA GGATGGAAGA GCTGGAATGA AGAGATGTCT GACAGGTCTA TTAGCCTTCT TCAGCAAGAA TGTGTGGGGT TTCCTGGTGT CAGAGTTGAA AAAGACTTGT GGGTTGGGGG CTATAGAGAT GGCTTGGTGG TTAAAATACT CTCTGCTCTT CCAGAAGACC TGAGTTCAGT TCCCAGAACC CATTCAGCAG TTCACACTCC AGGTCCAGGG GCTCTGACAC CCTCTTCTAG CCCTGTGGCT TCTGTAGGCT CATGTTAATG TACACACACA CACACACACA CACACACACA CACACACACA CATACACGAA GGAGCTCAGC CTAAACCCCG TGATCAGATA CTGCACCACA ATGGTAGTAG ATGTGATTGA GCTGAGGTGA CAAGCAAGAG GTGACAAAAA GCAGTAGCAT CCTTGCCTTT GTTAAGTCCT AATATTGTCG TGTGGATCTG AGAACCTATT AAGAGCTCAC CTTCTAAATG GATGCATGCA TTTCTCCCTG GGTCTCCTTG CCCACTGCTC TCTATCATCC TTGCGGCTGC ACCCTCCTGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_013547, NR_027778
Synonyms	aku; Hgo

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