XYLT1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	XYLT1
Locus ID	64131
Kit Components	KN222133G1, XYLT1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AATTTCAGCAGCCTCGACTC KN222133G2, XYLT1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCTGCAGACGCTGGTCGTG KN222133RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet ATGAAGGGAT CAGACTGTGG CTCTGACACT CCTGAAATGT TTCTCACGTG TCCACCTGCC AACCGGTCTG GTACCGACAG AAGATCCCTG GACCGCGAAC TCCGGGGCTA GGAGCTTCCT CCACGCACTG CCTCCTTCCA GCGGCCCACA GGGAAAACAG GACCTCGGGA CCCCCTCTCT TCCTGACCTC CCCTGGATTC TGAGCGGGCC CACCCTCAAC CAAAGGTCCC CTGTTTCGCG GCCCCTGCCT CTCTTTTTCC GCCTTGGGAA TTGTCCCCCT CGCCCCCCAT CCTACTCCCA CTACGTCCTG AGGGGTGTCT GTCTTCTCTG ATCGCCCCCA CCCCCTTCCT TTCCCTCCTC CTTTTCCTCC CTCGGCTCCT CCCCCAGGCC CCGCCCTCTC CGCCTCGGCC CGCGTCCCCC CGGCGCCTTC CCCATCACCC TCCCCTCCAG CGGGGACAGG GGTGTGGGGA GGGGGGCGGC GGCGGCGGCC CGAGCGGGAG CCCGAGCGGC AGCCGGCGGC CGCGGGAGCT GCGGGGAGCG CGGGGGCGGC CCGGAGCGTG CCGGGGTCCC CGCGCCTCGC TCGCCGGCCG CGCTCCGAAG TCTGGTAAGA TGCCCTTTCG GCTCGCGCCC CGCGGCGAGG GAGGGAGGAA GGCAGCCCGA CCCCACGCCC CCCTAGCCCT TGAACTCGGT CATCAGACTG GGACTCTGCA AGCCTACCGA CTCCACATCG CCTCTTGCCC CGGGAAGCCG GGTGGGGTCC CTGCCAAGGC TCAGGGGTCG TGGGCTGCCC CTTTCAGAGT CTGAGCACGG ACCCCTTGGG GTCTGGGGGA AGTTTTCTCC AGCGGGGGCG CTTTGGGGGT TACCGGAGCC TGGCTGGGTT CCTGTTCCCC ATCACTCTTG CTGGGGACCC CAGGTGACTT GCTCCGTGCG TGTAGGTCTT GGTGACTCGC TCCCTGCCTG AGTGAGTGGA TCTCCCCGGA TCCTCAGCCG CTGTTCCTGG GCGGTTGTCG CAGCAGCATC TCTCTGGTTT TTGCCGAGCT GGGTGCAGAC AGTTGAGCCC TCTGTTCGTC CTGGCCTGCG ATGAGGGCTG AGCTGGGCGT CCCAAGTCCC CGACCATGCC CCTGAATCAA TACTCAGTCC GGGGTGTCTG GTCTTACTTT GGGTCTTCTG TTTCTCTTCT CTGGATGTTT GAGCCTTCCG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_022166
Synonyms	DBQD2; PXYLT1; XT-I; XT1; XTI; xylT-I; XYLTI
Summary	This locus encodes a xylosyltransferase enzyme. The encoded protein catalyzes transfer of UDP-xylose to serine residues of an acceptor protein substrate. This transfer reaction is necessary for biosynthesis of glycosaminoglycan chains. Mutations in this gene have been associated with increased severity of pseudoxanthoma elasticum.[provided by RefSeq, Nov 2009]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.