IREB2 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	IREB2
Locus ID	3658
Kit Components	KN221385G1, IREB2 gRNA vector 1 in pCas-Guide CRISPR vector KN221385G2, IREB2 gRNA vector 2 in pCas-Guide CRISPR vector KN221385RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet TCATTCAACA GTTGAGCGCC TCCCACGCGC CCGATCAGTA TGGCCGCCCC CACTTGAAAA CACGCGTGTG GGCCGCCCAC GTCTGACAAG TTAATGCAAG GCTTTATAGT TAGGGGAAAG CGGTTCTCCT TGAGCTCTTT CTCCTAGCAG TTCCGATTCC GACCCTGACT CCAACAGACA CCTTGCGGGA ACGCAAACAC CGCTCGAATT CATGACCCCA ATAGAAAACT TAAGGCCGCA ACTAAGTAAC GGATCGCTGC GAAGGCCAAA CTAGCCACGC CAACGCCCCC ACTGGAGCCT CCCCAGCGCT CCGCCCCCGC TCGCGAGAAC AGCGGCGACG GCGCGAGAAA TCGCTTTCTG GTTAGCTCCG CCCCTTCCCT TTCTTTGTTT TCCTGTCCGA CGATCTCGCG GGAGTTAGGC GACAAATCCC GCGAGCGCAG ACCCGGGGCT GGCTCTGCTG CTCTCGCGAT ATTTGCGCGA GCCTGCTTCC TTCTTTCCTC CCTTGCCAGT CCGCCTGTCT TCCTCCCCGT CTTCCCTGCC CGGCCTCCCC CTTCTTCCCC CGCTGGCCCC CTCCCCGGAG GGATAATATG GTCTCCGGCG AATCAGTCCC TCTAATGTTA ATTGTGGAAT TCACAAATTC CTGTCTCTGC AGTTTTCAAG ACATGTCTTT CCAAAACGTC CCCGTGGATT ATTTAAATCA TTCTTATTGT CCCTTTCCAG GTATTCTTAT CACATTTCAG GAGTGAATTA TCTTACTAGT TTTTACTTGA CAAATTATGG TTTAGAACTA TGCTTTCCAG TGTGGTGGAT ACTAGCCCTG TGTGACTTTA AATTAATCAA AATTAAATAA ATTAAAAATT CAGTTCCTCA GGCACGATAG CCACATTTCA GGTGCTCAAT AGCCACACGT TGCTAATGGC TACTTTACTG GACAGCTCAA ACATAGAATA TTTTTACCAT TGCAGAAAAT TCTATTGGAT AAAGCTAGTT TCCAGGTATT TTCAGCTGAA TAAAATATTT TGTTGCTGCA TTTAATGAAA ATACAATTTT TTTTTCAGGA TACGCCTTTG AGTACCTTAT TGAAACATTA AATGACAGTT CACATAAGAA GTTCTTCGAT GTATCTAAAC TTGGCACCAA GTATGGTAAT GTTGCTTTAC ATTTTCTTGG GTTTGTTTAG TCTCTAATAA TGAAAATGCA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001320941, NM_001320942, NM_001320943, NM_004136, NM_001354994
Synonyms	ACO3; IRP2; IRP2AD
Summary	The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

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