Growth hormone receptor (GHR) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Growth hormone receptor
Locus ID	2690
Kit Components	KN218710G1, Growth hormone receptor gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ACTTGATCCTGCCAGTGCCA KN218710G2, Growth hormone receptor gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTCGCTACATCCAAGTTTTT KN218710-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TTAGTTTTAC TAGGGAGTTT TTGTTTCCTT TTGCTTCCCT CCTTCTTTCT GCTTCCCTTC TAGTTAAACC TCTTTAGGAT GGCTCATTAG CAACTCGTTT TGAGTGGTTT GAGCTCTTTT GTTCACTGGG AAACAGATTT ATGAAATGTT ACTATTACCA TGATTGTTAT GTTTTTCCTT TTATGGCCTG TCCAGCTCAA GGCCCCATGC TTTCTGATAC TGCTGATAAG GTTTTCTATT TCCAGATCAA ATTAAAGCAA ACCTTACTGG CCCTGTTACT GAAGCTGTGC ATGGGGGTCG TTTGCTGTGA GGTGTTTCTA TGGCTTTGAG CCAGGGTATG AACATCTCTA GTGTTCATGT TATTTCCTGA GACTAGCACT CACGGGAAGT AGAATTTATT ACAACCTGCT GTTTGAGTTC ATGAAAAGTA GGACAATATG AGACTCTGGG GCAGTGAAAG ACTTACCAGG ATTCCTTCTG GAACTGACTC GTCAGCTCAT TCATGTCTTA CCCAGTCTTT AAACAGTATT TCATGATAAT GGTCTGCTTT TAATTGCTGG GCTTTACCTT ACCCTTTTTG TGATTGCAGG TCCTACAGGT GGATGATTTT ATTAAAAGAT GCAAGTTTTA CATAGCAGCA AAAAGGAAAC TTGACTTAGC TTTAAAATCA GAAACTAGTA GGACTTTTCT GTGGATGTTT AGGAGGAATG CAGTAGTCAG TCAACTTTGG CATCTGATGT TTTCATATCA AAAATTAATA CTACAAATCA TGCTGAGGAC ATTTATGTTG AAGACAAGCC AGGTCTCTTG TTCTCAGGCT TTGGGAGAGA TATTGAAATT ACACTGAAGG GTTATTAGCT TACTGTTCAC TCAGCTCATC TGTGACTAAG GATATCTAAT CTATTTCCAG AGCTCAGAAT TCAGGCTTGT TAATCATATT CTCAGAGAAA AGAAATTGCA GGTTTCAAAT GAATTTCACT TCTCACTCTC TGGGTGATTA CCATTTGGCT AAGTTGAGGA AAGAGACACC TGAAATAGTT GAATGATATA CCCAGAAATT ATGAGCTTCC TTCCCCATCT CTATAATTCT CCCTCCCTTT TTTATATCCC TCTCCTTGTA GAGAAGTCTC AATATGTTTA AACTATGTTT CATAGCAGAC CTAACTAAAA CAAGGAAGAG TAATAGAAGG GAAAGGGAAG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000163, NM_001242399, NM_001242400, NM_001242401, NM_001242402, NM_001242403, NM_001242404, NM_001242405, NM_001242406, NM_001242460, NM_001242461, NM_001242462
Synonyms	GHBP; GHIP
Summary	This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

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