Growth hormone receptor (GHR) Human Gene Knockout Kit (CRISPR)

CAT#: KN218710

GHR - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Anti-GHR Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


GHR (Myc-DDK-tagged)-Human growth hormone receptor (GHR)
    • 10 ug

CNY 7,128.00
CNY 7,505.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol Growth hormone receptor
Locus ID 2690
Kit Components

KN218710G1, Growth hormone receptor gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ACTTGATCCTGCCAGTGCCA

KN218710G2, Growth hormone receptor gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTCGCTACATCCAAGTTTTT

KN218710-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TTAGTTTTAC TAGGGAGTTT TTGTTTCCTT TTGCTTCCCT CCTTCTTTCT GCTTCCCTTC TAGTTAAACC TCTTTAGGAT GGCTCATTAG CAACTCGTTT TGAGTGGTTT GAGCTCTTTT GTTCACTGGG AAACAGATTT ATGAAATGTT ACTATTACCA TGATTGTTAT GTTTTTCCTT TTATGGCCTG TCCAGCTCAA GGCCCCATGC TTTCTGATAC TGCTGATAAG GTTTTCTATT TCCAGATCAA ATTAAAGCAA ACCTTACTGG CCCTGTTACT GAAGCTGTGC ATGGGGGTCG TTTGCTGTGA GGTGTTTCTA TGGCTTTGAG CCAGGGTATG AACATCTCTA GTGTTCATGT TATTTCCTGA GACTAGCACT CACGGGAAGT AGAATTTATT ACAACCTGCT GTTTGAGTTC ATGAAAAGTA GGACAATATG AGACTCTGGG GCAGTGAAAG ACTTACCAGG ATTCCTTCTG GAACTGACTC GTCAGCTCAT TCATGTCTTA CCCAGTCTTT AAACAGTATT TCATGATAAT GGTCTGCTTT TAATTGCTGG GCTTTACCTT ACCCTTTTTG TGATTGCAGG TCCTACAGGT GGATGATTTT ATTAAAAGAT GCAAGTTTTA CATAGCAGCA AAAAGGAAAC TTGACTTAGC TTTAAAATCA GAAACTAGTA GGACTTTTCT GTGGATGTTT AGGAGGAATG CAGTAGTCAG TCAACTTTGG CATCTGATGT TTTCATATCA AAAATTAATA CTACAAATCA TGCTGAGGAC ATTTATGTTG AAGACAAGCC AGGTCTCTTG TTCTCAGGCT TTGGGAGAGA TATTGAAATT ACACTGAAGG GTTATTAGCT TACTGTTCAC TCAGCTCATC TGTGACTAAG GATATCTAAT CTATTTCCAG AGCTCAGAAT TCAGGCTTGT TAATCATATT CTCAGAGAAA AGAAATTGCA GGTTTCAAAT GAATTTCACT TCTCACTCTC TGGGTGATTA CCATTTGGCT AAGTTGAGGA AAGAGACACC TGAAATAGTT GAATGATATA CCCAGAAATT ATGAGCTTCC TTCCCCATCT CTATAATTCT CCCTCCCTTT TTTATATCCC TCTCCTTGTA GAGAAGTCTC AATATGTTTA AACTATGTTT CATAGCAGAC CTAACTAAAA CAAGGAAGAG TAATAGAAGG GAAAGGGAAG

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000163, NM_001242399, NM_001242400, NM_001242401, NM_001242402, NM_001242403, NM_001242404, NM_001242405, NM_001242406, NM_001242460, NM_001242461, NM_001242462
Synonyms GHBP; GHIP
Summary This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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