IQGAP1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	IQGAP1
Locus ID	8826
Kit Components	KN218549G1, IQGAP1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGCCCGTCAACCTCGTCTG KN218549G2, IQGAP1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ACGAGGCGCGATTTTCCTGG KN218549-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TGAGGCCAGG TTGCCAAACT GCGGAGAGTG GGGTTGGAGG GCTTTCATGG CAGTTTGGGG CCTGCTTGTG TCCAACAAGA CAACCTGGGT CACCTCAAAG GGTGTCTTCT CCAAATATTG GTGCAGAGGT TAGCTGGGCC GGCAAGAGAA GGGAGATGAC CCACTATTTT GATTTCAGGC AGCAGACACC GGCAGCCTTT CGGTTAGAGT TGCGGATAGG GCAGTGGCCT TGGGGGCGGG GCTATAAACC TGGGCGGGCA TTCCCGGCAG GGCAAGCTCA GCGTGAGCCC GATTGGAGGA TGGGCTCTAC CTTCTTCCTT CCGCCTACGG AGACCATAGC GGGATGCAGG GCGGGGCCGG GCTGAGGGGG CGGAGTTAGG GGCGGGGCAC GGTGACTCTG GCCCCGCCTC TTTCCGGCCG CTCAGGGCGG TGTCGCTGGG GCGGTGCCGC TGGGGCGGGG CCTGCGGGGT GGGGCTGACG GCACGGGGCG GGGCCTCGGG GACCCCGGCA AGCCCGCGCA CTTGGCAGGA GCTGTAGCTA CCGCCGTCCG CGCCTCCAAG GTTTCACGGC TTCCTCAGCA GAGACTCGGG CTCGTCCGCC GGGCTCGGCC GGGCGGGTGG GGCAGGGCGG CTGCCGGCAG CTCGGACCCG GAAGAGCCGT CCCGGTGGGG CGGCGGGCGG CGGGCGGGGA GGCCCCTGGT TCGCGCCCCC TCGGGGTCCG AGGCGGCGGA GAGGACCCCC GAGGCCTCCC GCCTTAGCTG GGGCCGCGGT AGGGGGAGCC CCACCCAGCG CCTCTCGCCG CCCAGGCTCG GCCGAGATTG GGGAAGACGG CCTAGAGTGA GGTGGCAGGA TGACCGCGCA GGGCGTGTCC CGGGGATTGG GGGTCTGGGT ACGCGGGCTC TGCCCGTTTC GTGGAAAGGC GCTGCCCTAG TGTTGCCTGC GGGGTAGACC CAAGGGATAG ACCAGGGTCC AGACCTGTCC GGTCCCAGGC TAGCTACGAG GTGCCGTCCT GGGCAAGTCA CTTCAGTTTT CTCGGGCTTA GTTCCCTCCT CAAGATTTCA AAAGTAAAAT AGTAATAAAG AAAAAAAAAA GAAGCAGAAA ATCCTCTGAC TTCCTCATTT TCTGTTGTCG GCCCTGGACG TCATTGAACT GCAGGCAGGA TTGGCTAAAG GAGGACCCTT TGGGATTTTG AGGTTTTCGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_003870
Synonyms	HUMORFA01; p195; SAR1
Summary	This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]

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