CD22 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA	Luciferase-Puro
Symbol	CD22
Locus ID	933
Kit Components	KN216939G1, CD22 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTGTGGCCTGGGCTAGTACT KN216939G2, CD22 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGGGCTAGTACTGGGGTTCT KN216939LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. Homologous arm and Luciferase-Puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet GGATGGGAAA AAGCAAGCAA GGGAGGAAGG AAGGAAGAAA GGAAGGAGTT CTGGGAAGAG AGAGAGGCTG CCCCCGCAGG GCTCTCAGTT TGAAGGAGAG GTGCAGCACA CTCAGAAAAA ACTCCCGGCC AGGAGTGGTG GCTCACACCT GTAGTGCCAG CACTTTGGGA GGCCAAGGCT GGCAGATCAC CTGAGATCAG GAGTTCAAGA CCAGCCAGGC CAACATGGTG AAACCCCATC TCTACTGAAA ATACAAAAAT CAGCCAGGCG TGGTGGCACC TGCCTTTACC CAGCTACTTG GGGGGCTGAG GCCAGAGAAA CGCTTGAGCC TGGGAAGCAG AGGTTGCAGT GAGCGGAGAT CACACCACTG CACTCTAGCC TGGGAGACAG AGCAAGACTC TGTCTCAAAA AAAAAGAAAG AACTCCGTGA GCATCCCAAA TGCCACATCC CCATAATGCA ACCAGACCCG GTCCTCCCGG ATCCAGGGGA AGGGTCGGAG CTCAGTGGGT GAGCAAGAAA AGCCACGTGG AAGAAGTAAG CGGCCAGATG GCTCAAAGAC AAACTCTCCC CTGCTCAGGC TTGCACCCAG ACACGACACC ACATAGGGTA GGGTGGGGGC AGCGGTGTGT ATAGATAAAT GTACATACAA ATATATATGT TTCCCATATA TACATACACT TCTCTCATTG CATCCTGCTG TCTTTGTGTA TCTCTGTATT TCTCCCACTA TCTTCAGAGG GAATTCTTTA GTCTTTAGAA AGCTCTCTGC TGGGTGCAGT GGCTCATGCC TGAAATCTCA GCACTTTGTG AGGCTGAGGT GGGAGGATCA CTTGAGGTCA GGAGTTCAAG GCCAGCTTGG ACAACATAGC AAGACCCCTA TCTCTAAAAA GAATAAAAAA TTAAACAAAT TTTAAAATAA AAAATAACTT TTAAAAGAAG GCTCTCATGC CCCCTTAGTA ATGCTTTCTG ATCACTGTGG TGAGTTCTAG AATACTTGGC TTTCTCTGAC TCAAGTAAAT GGGTTTTTGA GCACCCTGAA ACCCTCTACG CCTGGGAGGG GGCCTGCGTC TGGATCCCCT GCACCTACAG AGCCCTAGAT GGTGACCTGG AAAGCTTCAT CCTGTTCCAC AATCCTGAGT ATAACAAGAA CACCTCGAAG TTTGATGGGA CAAGACTCTA TGAAAGCACA AAGGATGGGA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001185099, NM_001185100, NM_001185101, NM_001278417, NM_001771
Synonyms	SIGLEC-2; SIGLEC2
Summary	Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.[UniProtKB/Swiss-Prot Function]

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