CFTR Human Gene Knockout Kit (CRISPR)

CAT#: KN216476

CFTR - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
4周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Rabbit Polyclonal anti-CFTR Antibody
    • 100 ul

CNY 1,999.00
CNY 2,700.00


CFTR (Myc-DDK-tagged)-Human cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR)
    • 10 ug

CNY 10,128.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol CFTR
Locus ID 1080
Kit Components

KN216476G1, CFTR gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAAGGTGGCCAACCGAGCTT

KN216476G2, CFTR gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ACAACGCTGGCCTTTTCCAG

KN216476-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

GAGGAGGAAG GCAGGCTCCG GGGAAGCTGG TGGCAGCGGG TCCTGGGTCT GGCGGACCCT GACGCGAAGG AGGGTCTAGG AAGCTCTCCG GGGAGCCGGT TCTCCCGCCG GTGGCTTCTT CTGTCCTCCA GCGTTGCCAA CTGGACCTAA AGAGAGGCCG CGACTGTCGC CCACCTGCGG GATGGGCCTG GTGCTGGGCG GTAAGGACAC GGACCTGGAA GGAGCGCGCG CGAGGGAGGG AGGCTGGGAG TCAGAATCGG GAAAGGGAGG TGCGGGGCGG CGAGGGAGCG AAGGAGGAGA GGAGGAAGGA GCGGGAGGGG TGCTGGCGGG GGTGCGTAGT GGGTGGAGAA AGCCGCTAGA GCAAATTTGG GGCCGGACCA GGCAGCACTC GGCTTTTAAC CTGGGCAGTG AAGGCGGGGG AAAGAGCAAA AGGAAGGGGT GGTGTGCGGA GTAGGGGTGG GTGGGGGGAA TTGGAAGCAA ATGACATCAC AGCAGGTCAG AGAAAAAGGG TTGAGCGGCA GGCACCCAGA GTAGTAGGTC TTTGGCATTA GGAGCTTGAG CCCAGACGGC CCTAGCAGGG ACCCCAGCGC CCGAGAGACC ATCATTCATT GTTTTGAAAG AAAATGTGGG TATTGTAGAA TAAAACAGAA AGCATTAAGA AGAGATGGAA GAATGAACTG AAGCTGATTG AATAGAGAGC CACATCTACT TGCAACTGAA AAGTTAGAAT CTCAAGACTC AAGTACGCTA CTATGCACTT GTTTTATTTC ATTTTTCTAA GAAACTAAAA ATACTTGTTA ATAAGTACCT AAGTATGGTT TATTGGTTTT CCCCCTTCAT GCCTTGGACA CTTGATTGTC TTCTTGGCAC ATACAGGTGC CATGCCTGCA TATAGTAAGT GCTCAGAAAA CATTTCTTGA CTGAATTCAG CCAACAAAAA TTTTGGGGTA GGTAGAAAAT ATATGCTTAA AGTATTTATT GTTATGAGAC TGGATATATC TAGTATTTGT CACAGGTAAA TGATTCTTCA AAAATTGAAA GCAAATTTGT TGAAATATTT ATTTTGAAAA AAGTTACTTC ACAAGCTATA AATTTTAAAA GCCATAGGAA TAGATACCGA AGTTATATCC AACTGACATT TAATAAATTG TATTCATAGC CTAATGTGAT GAGCCACAGA AGCTTGCAAA CTTTAATGAG

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000492
Synonyms ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1; MRP7; TNR-CFTR
Summary This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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