CCR5 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CCR5
Locus ID	1234
Kit Components	KN216008G1, CCR5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TAATAATTGATGTCATAGAT KN216008G2, CCR5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTTCACATTGATTTTTTGGC KN216008-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GGAGGATCGC TTGAGCCCAG GAGTTCGAGA CCAGCCTGGG CAACATAGTG TGATCTTGTA TCTATAAAAA TAAACAAAAT TAGCTTGGTG TGGTGGCGCC TGTAGTCCCC AGCCACTTGG AGGGGTGAGG TGAGAGGATT GCTTGAGCCC GGGATGGTCC AGGCTGCAGT GAGCCATGAT CGTGCCACTG CACTCCAGCC TGGGCGACAG AGTGAGACCC TGTCTCACAA CAACAACAAC AACAACAAAA AGGCTGAGCT GCACCATGCT TGACCCAGTT TCTTAAAATT GTTGTCAAAG CTTCATTCAC TCCATGGTGC TATAGAGCAC AAGATTTTAT TTGGTGAGAT GGTGCTTTCA TGAATTCCCC CAACAGAGCC AAGCTCTCCA TCTAGTGGAC AGGGAAGCTA GCAGCAAACC TTCCCTTCAC TACAAAACTT CATTGCTTGG CCAAAAAGAG AGTTAATTCA ATGTAGACAT CTATGTAGGC AATTAAAAAC CTATTGATGT ATAAAACAGT TTGCATTCAT GGAGGGCAAC TAAATACATT CTAGGACTTT ATAAAAGATC ACTTTTTATT TATGCACAGG GTGGAACAAG CAAATCGCAG CCCGCCTCCT GCCTCCGCTC TACTCACTGG TGTTCATCTT TGGTTTTGTG GGCAACATGC TGGTCATCCT CATCCTGATA AACTGCAAAA GGCTGAAGAG CATGACTGAC ATCTACCTGC TCAACCTGGC CATCTCTGAC CTGTTTTTCC TTCTTACTGT CCCCTTCTGG GCTCACTATG CTGCCGCCCA GTGGGACTTT GGAAATACAA TGTGTCAACT CTTGACAGGG CTCTATTTTA TAGGCTTCTT CTCTGGAATC TTCTTCATCA TCCTCCTGAC AATCGATAGG TACCTGGCTG TCGTCCATGC TGTGTTTGCT TTAAAAGCCA GGACGGTCAC CTTTGGGGTG GTGACAAGTG TGATCACTTG GGTGGTGGCT GTGTTTGCGT CTCTCCCAGG AATCATCTTT ACCAGATCTC AAAAAGAAGG TCTTCATTAC ACCTGCAGCT CTCATTTTCC ATACAGTCAG TATCAATTCT GGAAGAATTT CCAGACATTA AAGATAGTCA TCTTGGGGCT GGTCCTGCCG CTGCTTGTCA TGGTCATCTG CTACTCGGGA ATCCTAAAAA CTCTGCTTCG GTGTCGAAAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000579, NM_001100168
Synonyms	CC-CKR-5; CCCKR5; CCR-5; CD195; CKR-5; CKR5; CMKBR5; IDDM22
Summary	This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015]

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