ALG12 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	ALG12
Locus ID	79087
Kit Components	KN215501G1, ALG12 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGCCGTAGCCACTGTCCACC KN215501G2, ALG12 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATCTGTCCCTACACCAAAG KN215501-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GGGGGCTTTG CTGGCCACTC GAGCCCTGTG TCCCAGCAGC TGTACTACGT TCAGTTTCTT GCCCTCCCGG GGCCCCTGTC CACCACTCTC CAGCTTGGTG CCTCCCACCC AGCTAACTGG CACCCCTCAG CCTCCCATTC CGTCTTCTTC AGCCGGTTCC CTCGGGGCCA AGCCTGGGTT TTGCTGACTC TCCTGCCAGA TTTAGACTGG GGGGCAGGGG CCATTTACCA CCCTCCATTG CCCTCGTGGC CCAGGCCTGG TCAGACTCAG GGTTCTGCGA CTGAGATCCT TCTGCCTAGA AAAACATTCA CAGAGCTGGA AGTGTCAGAT CACTGGTTAC AGTATGAAAG ATTCTCAAGT GTGATTTTTT CTCCAAACTT TCCATCTTGA TGCGTTTTCA GATTTGTAGT AGTGCGACTA AGACAGTAGT TCAGAGAATT CTGCTAACCC TTTACCCGGA TCTCCCAAAC GTGAGCGCAA GCGTGACTTT CGAACCTCAG GAATCTGTTT CCACTTTTCC AGCATGCGTG GAGTGGCAGT GCTAACGGCT GGTGTCTCGC ACTGTTGGCC TGTGAAGGTA CGTGAAGCTG AAAGCCTGGA TGGAGGAGAG CTTCAACCTG CAGGCCACAC ATGACCTGCT CTACCACTGG CAAGACCTGG AGCAGGTGAG GGGCCCCTGC TGTCTTTGGG GAAAACCTCA TGTTACCAGC TGGCAGGGCC CCGGTGCACT GACCTGCCCT TCTCCTCGCA GTACGACCAT CTTGAGTTCC CCGGAGTCGT CCCCAGGACG TTCCTCGGGC CAGTGGTGAT CGCAGTGTTC TCCAGCCCCG CGGTTTACGT GCTTTCGCTG TTAGAAATGT CCAAGTTTTA CTCTCAGCTA ATAGGTAAGG GGGTCTTCAA CAAAGGAGGA GGGAGTGAGG GACTGTGGTG TCATCGGGTG ACCTCAGCGA GGCCGCGGTC CCTCCTGCCC AGGAACGCTG TCTGTTAGTC TTGCCTGCCC TCGATCACCG TGGCATGGCG GCTCAGAGCA GGTGCTCAGG TCAGGCCTAA GACGTCTGAG TCAGCCCCTC TGTGCCTCCA CACTCTTGAC TGTGAAATTA GGGGTGACGG CAGGTACAAG AGAAATGCCG ACTGCCTGTG AGGCAGCTCT CGCCACCTGA GCCTGCTGCT CCCGCCTCAC CTGGCAAGCC CCCATGGGGT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_024105
Synonyms	CDG1G; ECM39; hALG12; PP14673
Summary	This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation. [provided by RefSeq, Jul 2008]

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