PIK3CB Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	PIK3CB
Locus ID	5291
Kit Components	KN215433G1, PIK3CB gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CATCCTTGACATCTGGGCGG KN215433G2, PIK3CB gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ATCTGATGGCTCCATACCTG KN215433-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCTCAAAAAA AAAAAAAATT GTCCTTGTAG AGAAAGGGCC CTCTTGATTG CAAATTGTTC TTGTTGAGAG AGGGCCCTCT TGATTGTCAT TTTCATTGGA ATTTGCAGAG AAAAACTCAG CCTAAGAAGC TAATATTTTC TAAATAAAGT TTGGAGAAAA ATCACCTGTC AAGTGCTGGT TAACTAAATT TCTTAGGACC CTGTAATCCT TAAGCATGCA GTTTATTCAG ATGTCAAATA TCTATGTTGA ATTTGAATCA ATGTGGTGTG TGTGTGTGTA CATATGTATG TGTATATATT TTTATTTATA TGTATATAAG AATAATTTTT TTCCTTTAGT TGGTGGTATG GATTGGATAA TGTACATATA TATAATTTGT GAAGTGATAA AAGTGTATTT AAATAATTTA TTTCTGCTGA CTTACTCTTG GTTTTTAAAA TATCACATTA TTTAATTGGC CTCCTGTTAC ATTATTGGCT ATATTGAAAT CTAAGGACTA ATTGTAGATT AGTGGTCTTT ATCATATCTT GGTATAAAAT TTGGCTGATG GTTGTTTTGC TATGTTTTAA TTACTGATTT CTAGGGCCCC GTGGTTATGA GGGATTTATA TCCAGTTGGA GGTACCTCGG GAAGCTACCA TTTCTTATAT TAAGCAGGTA TGTTAATAGG TATAATTTCT ATGTGTTTAG CATACTGTGT CACTTGGGGG AAATATCATT CTATAATAGA AAATGTTAAT GTAAATTATA TGTGATAATG ATAGTAATGA AGTGATGGTA ATTAAGAAAA ATTAGTTTAT GTTAGCTAGT TTGAAATGTT TGTTTGGGTT ATTAAAACTG TCAAGAAGAT TTTCTTTTGA GTTCGTCTTT GAAATTACTG TCTTTGAGCT TGTCACACTT TTAAAAATGT GATTTTTGGT TCATTTTCTC TTCTCTCCTT TTTCTTCTCT TCCCCCTTTC CTTGACTCTT GGGGGAAGAA AGACTTTTCT GCTGTTCACC AGATATTTTT CCCTCAGCAG GCTTGCCTTT TCTGTTGCAT TAGTTTTTAA AGTAAATTGA TGAGCACCGT TGATTTGTAA AAACCAGCAT ATACATGAAC TTGTTTTTCT AAACTTTCTT TCTAATGTTT TTCAAAAAGC TGAACCCAAT GTGAATATCA GGAGTTACGG TATTTTTAAG AAAACATATT TATGATATAC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001256045, NM_006219
Synonyms	P110BETA; PI3K; PI3KBETA; PIK3C1
Summary	This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

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