IRE1 (ERN1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	IRE1
Locus ID	2081
Kit Components	KN215023G1, IRE1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTCCCCGGTCACTCACCCCG KN215023G2, IRE1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGGTGGAGGACAGCTCGCGA KN215023-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TTCGCTCCGG AGGTCGCCTC GGTAAACCTT GCGGATGATT CCACCACGGC GGAAAGACGA AAAGGAGGCT TCTAGGCACC CGGGACCGCC CGTGTCCCGC CCTTTTCCCC GATGAAGTCT GACGCGGCAG GTCCCTCGGC AGGGTTAATG GGACGGCCTG GTGGGGCATT GCCGGTCTTT CCCAGCCACA GACAAGCTTC GCGCCTCGAT CCCCCAGCGC CCCCAGACGC TGGATGCCGA GGCCTGGTCC TTTACAGCCG CCGACGCCTC ATGAGCGAAG CCAGCCAGAT GCCAGCACCG CCTTCGGCCC CGCCCCGAGC CAGCCCCTCC CACATCCCCG CCCGGGGCAC GCCCTCGGCC CGCCCCCATC TCCGCCCCGA ACACGCCCCT AGATTGGCCC CGCCCCGTCC CCGCCCGCAA CTCCGCCCCG ACCGGAAGAA GCCGCGGCAC CCAGCGCTTA TAGGGCCGGG AATGGGGGTG CCGCCGCTGC CTAGTCAGTT CTGCGTCCGC TGAGGCTCGG TCACCGCCTC GCTGTCGTCG CGGCGCCCCC GCCCCGTCCT CTGTCCGTAC CGCCCCCGGA GCCAGGGCCG AGTCCTCGCC CGGGGCAGCG TCGCGGCGCC GGGGCTGGAG TCCGAGCTGG GAGCGCGACG GCGGCGGCCA GATGCCCCGT GAGCCCAGCC GTGGGAGGCT CGGGCCGGGC GTAGGAGGCG GCACCGGGCC GGGAGAGCTT CCCTGACGGG AGTCGCGGCC CCACCGAGGC GTCGGCCGCC CGAAGAGCCT CCCAAGCCAG CGCGGCTGTC ACGGGCAGCG GCTCCCCGGA CCCCGCGGGC TCGGGCGCAA CTTCGTGGCG TCCTGCACGG CACTTGGGGG TCCCAGAAGG CCCCGGCCTG AGGAGGCCGC CCACCTGCGG GACCCTCCTC CTCGCGGGCG GGCGCCGGCG CTGCCTGGCG GGGGCCGCGG AGGAGCCGAA AGGGCGTTGG GATCCAGGGG AAGGGTCTGT CCTGCGCTTC GAATCCTTGT TTGAACTTTT TAAAAATTCG AAAACTTTGG GGAACGAATG CAACAGGGAG TAAGGACTCT CACCCGAGAG CTGGCTTCTG CCACTTGCCC TGCCGGTTTC TTGGTACTGG GAGGCTGCAG GCTTCCTTCT GTGCACCGAG ACTTCCTTGA GCAAGTGGAG CTGTGGGAAT TGCACTCTTG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001433, NM_152461
Synonyms	hIRE1p; IRE1; IRE1a; IRE1P
Summary	This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]

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