RTEL1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	RTEL1
Locus ID	51750
Kit Components	KN214507G1, RTEL1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGGCATTTGTAGGGCTGGAA KN214507G2, RTEL1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGTCCTGGAATGTCTGCAGC KN214507-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CTCCAGCCAG CCAGGAGGGC AGAGGGCTGG AGAGGCAGGG CCGGAGGTTC AGACCCTCCG CTCTGACGTT GCGCCTGGTG AGGCCGGGAG GGGTGCCGCT TGCCTCTTCA GCCCTCACGC TCTTGTGGAA GTCGCGGAAT TACTGCAGGC GGAACTTGCA GCACTGTGGG CGTCTTTTCC AGAGAAGGAC GGAGTTGTGG GGCGGGAGGA TAAGGCAAGG CCCAGCCACT TCGCATCTTC GCCCCGCCAG CTCCTCGAGA TGGGATATAC CAGGGTTGCT CTCCAACCCT CTCCGCAGGA GGGACTGATG GAAACGCCTG GGAAAGTAGC CCGGTACCCA CAAAGGCTGT CTACAAACAG AGTCTTACTG TCTTTCCCAG GTCTGTGCCA TAGGGATTCT CGAAGAGAAC AGCGTTGTGT CCCAGTGCAC ATGCTCGCAT CGCTTACCAG GAGTGCCCGA GACCCTAAGA TGTTCGGAGT GGTTTTTTCG CACAGACCCG AATAGCCTGC CCCTCAGCCA CGCTCTGTGC CCTTCTGAGA ACAGGCTGAT AGGTAGAGCA CAGGCCCCGA GGAAAGGACT GCGGGTGGGT GGAGCTTCAG CCAGGACGGG GTGTGCTTCC CTCTCCCGGC CCATTCCAGC CAGGCCCCTC CGGGCCAGAG GCAGCGTCTG TCATAAAAAG GGCTGGTGTT CCAGGTGGGG TCAGAGAGAG GATTGACAAG TAAAAACGAT CGTCCTTTGA AGGGGGCCGG CCCCTCCACA CCTGTGGGTA TTTCTCATCA GGCGGGACGA GAGACTGAGA AAATGAATAA GACACAGAGA CAAAGTATAG AGAGAAAAGT GGGCCCAGGG GACCGGCGCT CAGCATACAG AGGACCTGCA CCGGCACCAG TCTCTGAGTT TCCTCAGTAT TCATTAATTA CTATTTTCAC TATCTCAGCA AGAGGAATGC GGCAGGACAG CAAGGTGATA GTGGGGAGAA GGTCAGCAAG AAAACGTGAG CAAAGGAATC TGGGTCACAA ATAAGTTCAA GGGAAGGTAC TATGCCTGGA TGTGCACGTA GGCTAGTTTT ATGCTTTTCT CCACCCAAAC ATCTCGGTGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001283009, NM_001283010, NM_016434, NM_032957
Synonyms	C20orf41; DKCA4; DKCB5; NHL; RTEL
Summary	This gene encodes a DNA helicase which functions in the stability, protection and elongation of telomeres and interacts with proteins in the shelterin complex known to protect telomeres during DNA replication. Mutations in this gene have been associated with dyskeratosis congenita and Hoyerall-Hreidarsson syndrome. Read-through transcription of this gene into the neighboring downstream gene, which encodes tumor necrosis factor receptor superfamily, member 6b, generates a non-coding transcript. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]

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