Dectin 1 (CLEC7A) Human Gene Knockout Kit (CRISPR)

CAT#: KN214107

CLEC7A - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Rabbit Polyclonal CLEC7A Antibody
    • 100 ug

CNY 5,808.00


CLEC7A (Myc-DDK-tagged)-Human C-type lectin domain family 7, member A (CLEC7A), transcript variant 1
    • 10 ug

CNY 3,600.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol Dectin 1
Locus ID 64581
Kit Components

KN214107G1, Dectin 1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCGACTCTCAAAGCAATACC

KN214107G2, Dectin 1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTCTGAAACAACAGCTATCC

KN214107-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TGTTGTTACA ACAATGCATC TCCTCATGCC ATCCCTCATT TACCTATCTA TATATTTCCT TTATTAAGCA ATGAGTTTCT TCATAGGTAT CATTTTTCCT TGTATTGTAA GCTCCTACCA ATTGAAAAGG TGGGCATCCA GTACCTGTTT ATGTAATTTA AAAAATACAT GAATAAATAC ATGAACAAAG TTATGGTAAA CTTGGAGCAC CAAAACTGAA ATAGTTTGCA TCGGTTAAAT TAATTAATGT CAGACTTAGT TGTGAGAGTA ATGAAGGCAG CATAAGACAT TAATGTATGC ATTTTTCTTA CATTTGGTCT GAAACAAAGA CTCATCTGCT ATGCTGCCTT TTAGTTTTCC TGAGTCAGCA GTCTCAGAAA CAGGATCTAA AGGTACAAAT GGAAAATTAC CACAGCATAG TTTCATTTCC TGCTCTTGAA TATCTGGTTG AACTACTTAA GCTTAATTTG TTAAACTCCG GTAAGTACCT AGCCCACATG ATTTGACTCA GAGATTCTCT TTTGTCCACA GACAGTCATC TCAGGAGCAG AAAGAAAAGA GCTCCCAAAT GCTATATCTA TTCAGGGGCT CTCAAGAACA CTCCCGTTTG AAAAGATATA AGGGGAGGCT AGGCATGGTG GCTCACACCT GTAATCCCAG CACTTTGGGA GGCCGAGGCG GGTGGATTGA CTGAGGTCAG GAGTTCGAGG CCAGCTTGGC TAACGTGGTG AAACTCTGTC TCTACTAAAA ATACAAAAAT TAGCCGGGCA TGGTGGTGCA CGCCTGTAGT TCCAGGTACT TGGGAGGCTG AGGCAGGAGA ATCACTTGAA CCTGGGAGGT GGAGGTTGCA GTGAGCCAAG ATCATGCCAA TGCACTCCAG CCTGGGTGGC AGAGCAAGAC TCCATCTCTG AAAAAAAATA AAAATAAAAA CAAAACAAAA AGATATAAGG GGAGAAAATT TGAGAGCCTC GTGATGTGTA TATTTTTATG GATATACTTT TAAAGGCTTC AGATGTCACT GAAGATAAAT TGAATGCACC CTAAACCTTT TTTCATGAAA ATTATTCAGT GACCAATTAT TAGTGTTTAA ATTAATTATG TCTGATGGCA GAAAATAGAA TTTCTCTTTT CTATAGATAT AGACATTTCA TCAGTGAGGA ATTGGTAATA AGTGGGATAA TACACAAAGA TAAAGGATTT

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_022570, NM_197947, NM_197948, NM_197949, NM_197950, NM_197951, NM_197952, NM_197953, NM_197954, NR_125336
Synonyms BGR; CANDF4; CD369; CLECSF12; DECTIN1; SCARE2
Summary This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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