RICTOR Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	RICTOR
Locus ID	253260
Kit Components	KN212982G1, RICTOR gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGGGCTTACCTCGTACTCGG KN212982G2, RICTOR gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTCGGAGGTTCTTCAGAGAG KN212982-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GAGAAGCCCT CCTAGCCGAG AGGGGCATTC TGAAGATCAA GGGCGAGGCT GGAAAACAAA CTTTGTTACG TGTTAACTTA GTTGGAGCAA AGCGATTCCA GGAAAGGAGT CTAAAACTGT GCGCAGCGTT TTTATAAAAA AATGTTTCAG TGGATACACG GGGGAGCGGG GCCGTCTATG GCAGGGCTTC AGAGCAAGCA GGAGACTGGC AGGCCGGCGA GAGCAGGTGG CCCGCCGGCC GCATCCCGGC CTCCTCCTCC CCGCTCGCCT GCATTTGGAC GACGGCTTCC GCGGCGGCCG GAGGGGAGCA TCCTCTACAC TGCCAACTCG CATCAATGGG ACTTTCCTCG TGGGAACTGC CCCTCCCGGC GGACGTGCCC TCCGGGTCCG GCGTGGGCGG GCGGGTGCCG CGAACTCCTT GCAGACCTTG GGTCGGCTCA GTGCTCCCTC AGCGCTGGGG CCGAGCCTGC ACCACCCGCC GCCGTCGCCG CCGAGGGGGC GGGGGAGGTG CCGCGGGGGG CGGGCGCGGC GCGCGGGGAG GGGAAGGGGT TCTCGCGCGA TTGGGCGAGG TTTCCGGTGT TGTGACTGAA ACCCGTCAAT CGCCCGGCGC CCGCCACCCC TGGCACTTGC CTTCCCCTGG CCCTGGGTTG GGGAGCGAGC CGGGGCGCTG GCCGGAGCCA GGGCAGCCGC AGCTGGCCTG CGAGCCAATC CTAATTGGGG CTGTGTGTCT GTTTCGCGCA GGGCGGAATG ACAGCGGCGA GGAGAACGTC CCGCTGGATC TGACCCGAGG TAACGCGGGG CGCCGCGGGC GCCGCGAGGG CGCGCTGCTG GGGAGGAGCC GGGTCCGGGG CTGGGGGCCA GAGGCAGGGG CGGGCCCCGC ACGGGACCGG CGGGGTGCGG GGGCCAGAGC CGGAGCCCCG CCTGCCGGCC CGCGGGAACC GAGCCCGGGG CCGCCTCGCC GGACCCATCC CCCTACCCAG CGCCCGCTTC CCTCGCCCGA GCCCTTCTCC GGGCCTCGGG CTGCTGCGGG TGCGGGGGAG CCCCGAGTGG AACTCGGCAG ACCCGGCGAC CCTCTGGGCT CGAGCTCCGG CAGAGTGGTT TGCACCTATC CCGGAGTCCG GCAGCCCAAG CTAGGGGCAG CTGGAGGGAG GTAGAGGCTG TTATTTAGGT TGCAGGAGGA TGTTTGAGGG AAGAGCGACC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001285439, NM_001285440, NM_152756
Synonyms	AVO3; hAVO3; PIA
Summary	RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]

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