GPR 174 (GPR174) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	GPR 174
Locus ID	84636
Kit Components	KN211205G1, GPR 174 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTAATTACACGTGTACCAG KN211205G2, GPR 174 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TACACTGTCATTCTTGTGCC KN211205-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AAGCTGGGCC AGGAAATAAT TGACTTATTT TCTTTTGCTT GCAGCTCTGC CTTTGTTTCC TACTTTTTAG CTTTTGACCT ATAGCTGAAA AAGTCCCAGA AAGCTGATCT TTCCAAGTTA ATCATCCCTG AAGAGTGGCG GACTCATTGT GCACTAAAGC AATCCTTTCT TCTATGTGAG CCAAAAAAAG AAAAACGGCC TGCCAGACAC CTGTAAAGAG AAGGCTATCG TCATATAGGA ATTTGAGGAA CTGCTATATG GTACATTCCT TTTCCAAATG GGAGAGAATA AGCTCTCATG ATGTCCCAGA GGGCCTTAAA ATAAACAAGA GGGGAAATTG TAACAGCTTG TCATCTGTGC TTGTACACTA GACTTCCATG TAGTTACTCT AGAGAAATCT AAATCAAAAA TGCAGATCAT TCTTTGAAAA ATCCCCAAAT CATACGTAGA GACTCAGACA GATGTGGTAA AAAGAGGAAG GTTATTTTAT ACTTCGTATC TCCAACCCAC TGGCAATCAA TCTTTTGGAA GGAACAGCAG TTGATTGTGA ATTTAGTTTT GAACCACCAT TAGGCAAAGA TAGTTTCTCT AGAGAGAATC TCATAGGGAA TATATTAGCC CTGTGGGTAT TCTATGGTTA TATGAAAGAA ACAAAACGAG CTGTGATATT TATGATAAAC TTAGCCATTG CTGACTTACT ACAAGTTCTT TCCTTGCCAC TGAGGATCTT CTACTACTTG AATCATGACT GGCCATTTGG GCCTGGTCTC TGCATGTTCT GTTTCTACCT GAAGTATGTC AACATGTATG CAAGCATCTA CTTCTTGGTC TGCATCAGTG TGCGACGATT TTGGTTTCTC ATGTACCCCT TTCGCTTCCA TGACTGCAAA CAGAAATATG ACCTGTACAT CAGCATTGCT GGCTGGCTGA TCATCTGCCT TGCCTGTGTA CTCTTTCCAC TCCTCAGAAC CAGTGATGAT ACCTCTGGCA ATAGGACCAA ATGCTTTGTG GATCTTCCTA CCAGGAATGT CAACCTGGCC CAGTCCGTTG TTATGATGAC CATTGGCGAG TTGATTGGGT TTGTAACTCC GCTTCTGATT GTCCTATATT GTACCTGGAA GACGGTTTTA TCACTGCAAG ATAAATATCC CATGGCCCAA GATCTTGGAG AGAAACAGAA AGCCTTGAAG ATGATTCTAA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_032553
Synonyms	FKSG79; GPCR17; LYPSR3
Summary	This gene encodes a protein belonging to the G protein-coupled receptor superfamily. These proteins are characterized by the presence of seven alpha-helical transmembrane domains, and they activate or interact with various endogenous or exogenous ligands, including neurotransmitters, hormones, and odorant and taste substances. This family member is classified as an orphan receptor because the cognate ligand has not been identified. [provided by RefSeq, Sep 2011]

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