ATG5 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	ATG5
Locus ID	9474
Kit Components	KN210563G1, ATG5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AACTTGTTTCACGCTATATC KN210563G2, ATG5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAGATGTGCTTCGAGATGTG KN210563-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GAGGAGAAAC TATCTGGAGT AGGAAAAGGT GAATATATTG GACGGGGCAG GGGTAAATGC GAGAATGATC CTTTGGAGTT GGACTGTCAG GATTCACAGG GTATAGGTGA AGCTCCTCTT TAGGTAAGCA GTAGACTCTT CTGGGCTTGA AAGACTGATG CAGATACAGT TTCATTTTGA CTGTGGTTCA GTACTTAAAT ATGCAATGAA TATTACTGGC ATATGTAATT TTGATCTGAT TCCTTTACAT AAGTAATAAT TTTACATTTA GAATTTGTTG TACTCTTTTC TTTGAATTTA TACTAAAGAT TGGTGATCAT CGTTTTTTTT TTTTGAAGTG GATAATCTTT ATGGCATGGA TTTTTTAAAA TAATCTTTTG TTTTCTAGCA GGTTCATTCA TCGTTGCAAG GATCTGACTA ATGCTCTTCA TTTTATTTTC AGTTTGGCTT TGGTTGAAAT AAGAATTTAG CCTGTATGTA CTGCTTTAAC TCCTGGAAGA ACTTGGAGGC CATTATGTAA CGTTTTAGAT ACAGCTAATT CATGCTTATT TTGGAAAAAA AATTAACACA GATAAGCATA AAGAAGGAAA TAATCACCTC TAATGCTACC ACTCAGAGGA CAAAACCACT GTCAGCATGT TGTCATGATG CGTTCTGGAC TTTGTTTTCC TTACATGTAT GTGGGTATGT TAATGGAATC ATCAGCTATG GTGCCTTCTT GACTTTCTTT TTCTTATGCA TATATTCATA TATAATAAAT GTATGATAAT GGAATAATAA GATGTGCTTT TTTAATAGGA AGAGATATTG AAAATAATAG GTTCAGATTG TATTCTTTTG TAGTTAGGAG ATTCTAAGCA ACTGTTTGTT AAAAGTGTTT TTATAGCTTA AAGAACATAT AAACATGCAC ACATACACAC ACACGATACG TTCACTGAGC TATTGTATGT TATATGTGAG CTAATATGTA ATATATATTT AATATATATT ATAAATTGCA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001286106, NM_001286107, NM_001286108, NM_001286111, NM_004849, NR_104402, NR_104403
Synonyms	APG5; APG5-LIKE; APG5L; ASP; hAPG5
Summary	The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

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