ethanolamine kinase (ETNK1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	ethanolamine kinase
Locus ID	55500
Kit Components	KN209732G1, ethanolamine kinase gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CACTGTTGCCGATCCGGGTC KN209732G2, ethanolamine kinase gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGCGGTCCAGCTCCGACAAC KN209732-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TTCTTCTTCA TACCAGACTC TAATCTGTTC TCCCTAGCTT CGAATTATTC ACTGTATGGT CGAACATCTT ACTCCACAAA TAACCTCTGA AGTGTTGCAG TTGTCTTACC CAAATACCTG AACTCAGCTT GTCCCGAGTA CTGCGATCCC GCGTACTGCT AATGGGAATA TAGTTAACCC AACTAGTATG AGGTCCCCAG TACACAGCAG CTCCAAGATC AAGGCGACCG AGTAACACTG CTTGCCTAGA CTCAGGCCAA GTTCGGAGAG TGATCCTGGG GTTATTTTAG AACGTGTCCC TTGCTTCCCA GAAAAATTCA GTCGTTAACT CAAGTCCAGA TGCCCAATGA CCATTCGGAT AAGGTGAGCT AAAGCTTCAA TGCCTTGAGG GGCTTGGCAG GGCTCGGGCT CATCCCGGAG AAGCAGCCCC GCTTTCCAGA CTAGCAGTTC CCAGGGATGG GTGTGTGCTA ATCGCAGGCC AACAAGCCCA GCGCGGTACA GAGGTCCCAT TGTGACCGGA GGCGAGAAAC CCCGCCTCGG CACCCTGACG CAGCGCAGGA CCCGCCCCGC GCGTGACGCC AGCGTCAGGC CAGCCCCGGC TCGCCCGCCC GTCCCAGCGC CCCCAGCCCT CCCGCGAGGG CGCCCCGGGA CGGAAGGATC CACCAGTCTG TCGGCGCCCG CCGTTCTCGT GGTCGCCGTC GCCGTCGTCG TGGTGGTAGT CTCCGCCGTC GCCTGGGCCA TGGCCAATTA CATCCACGTC CCTCCCGGCT CCCCGGAGGT GCCCAAGCTG AACGTCACCG TTCAGGATCA GGAGGAGCAT CGCTGCCGGG AGGGGGCCCT GAGCCTCCTG CAACACCTGC GGCCTCACTG GGACCCCCAG GAGGTGACCC TGCAGGTAAC GCCCACACCT CAGCTCTGGG TCTCTTATGC CCCTCACGCG GCTCTGGGGG TCCTCACCAC AATCGCCTCT GTCCCACGAT GACCGAGGAG GACCTAGGGC AACATCTCCT TAAGTCTATT GGGAAGTGAC CCTGTATTTC CCCTCACACC TAGGAGGGTC ACTCCCCCTT CCCGTCGCAG TTGCTCTTTG AGATTGACCT GAGGCACATT TTCTCTTTCT AGAAGCCGCT GCGTAAGTGA CGGACCCATC CACGGGGGGA GATTCCTGCT GATAGTTGCC CCTCCTCCCA CTCCCCAGTC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001039481, NM_018638
Synonyms	EKI; EKI 1; EKI1; Nbla10396
Summary	This gene encodes an ethanolamine kinase, which functions in the first committed step of the phosphatidylethanolamine synthesis pathway. This cytosolic enzyme is specific for ethanolamine and exhibits negligible kinase activity on choline. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

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