GFM1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	GFM1
Locus ID	85476
Kit Components	KN208239G1, GFM1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCAGGTACCGGAGCATAGAG KN208239G2, GFM1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGCGGCTCTGGGGCGCGGAA KN208239-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CTTCCACATG GACAGGACAT GAAATGTCTG ACATCGAACA ATAACAAATA ACATTTTGAC ATGAGGAAAA GTATTTGTAA TTGTAATTGA TAGTTCATTC ACTATAATTT GTCCACGTTC TTTTTCAGGC CATGTCATAT ATATACCTAT TAATATTTGT AATAGACAAT TTTGCTCTTT TCTACTTAAA TTGTTATATA CATTTCTCCA CATTGCTTTA TAGGCCTAAC AACCATGTTT AAATTCTGTA TCTTCATACA GAGGTAATAT TTAAAAAAGA AGACGAAGAC AAAAGGACTC CCTTCCCTCA CCCATATACC TATGCCGTCT TACTGGGAGC AAACACTTCT TTAGCTCCGC AAAGCGATTG CACTTTCTCT TCCAAGTCGG CAGACGGCGC GCGAGCAGCG TAGGCACTGC TCCGCCTCCA ATCCAGTTGC TCTTCTTTCT TTTCCTCACT CTCTTTTTCC TCGCGTCCTT TGCCCCGGAA GTGCTCTTAC AACATTGGCT GCCGGCGTGA CTTTGACCGC TTCCCGGTGC GTTACCGGCA GCTGAACCCA CCCGGCGCCA CGGGACTTTG ACGCGTGCTC TGCGCTTGCC AGGCTAAATC GGGACCATTC CCGGAACCTT GTGATCCCTT CTGGGCAATG GAAGGCCGTG ACACCCCCTG GGTCCTCATG ACTGACAGCT CCGAATACTG GCAGTCGCTC GTCAGTGCTG AAAAGCACCT CGGTGCCTGC ACATTAGCTC GTTAGCTCGT TTGTTTCGCT CTCTGGTATC CCTAGGGTTT AATCAACTAA AAGTGTCTGT CACTCAGATA CTTTGATATT TTATCCACCT TTTCTAAGGT TTTTTTTTTT TTTTTTTGCC TCTCTTTTCC CGTGAATAAT TGTGCATTTT GGATGTGCAG TCCTATAGGG AAAGGTTTAT ATTGTATCTT CATTCCATCT AATAGGGATC CCCATCCCCT GCCACCAGAG TAAAGTTAAA ACAAGCTTCA TGAATTGTTT TTGTTTGGAG GGAATTGGAA ATCTTTGGGG GCTGTCTTGG AGCTACTAGC ATCACTGCAT AGAGCATATA AGTTCTCAAA CATATTTGTC ATCTGTGAAC TACAATAAAG GAGAAAGGGA AAAAAATTAA GTTAAATTTA AGCTAAAATT TGTATTTTCT ATTAAAGTAT TTTTTGGAGC TGTCTTTGTG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001308164, NM_001308166, NM_024996
Synonyms	COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1
Summary	Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. [provided by RefSeq, Jul 2008]

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