CCR6 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CCR6
Locus ID	1235
Kit Components	KN207722G1, CCR6 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGCTGAAATTCATTGATTCC KN207722G2, CCR6 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTTTGGCAAGGGGTATAATT KN207722-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CTGACATATA TTGTGAACGA TTTAAAGCCC GGGACTATGA ATTATACATT TTCTATGCGC TTTCACACTT CTATAGATTT TGTTCAGTAA AATCTTGTTG AATGTTTTTA AATTTTGCGT AAAGATTTTT GTGCAGATCA AAACTTGTGG ATTTCTGAGA GCACCTTATT TAACCAATAG ATCAAGGGTT GGCAAATTAT AGCCTCTAGC CAACGGACCC CGGCGAGGCC TGTTCAGTTA TGTAATTGTA GATGCTTTCA AGCTCCAAAG GCAGATTTGA GGAGCTGTGA CAGAGACCAT ATGGCCTGAA AAGCCCTAAA ATATTTACTA TCTGGCCCTT TACAGAAAGA GGTTGCTGAT GCCTATTCTC AATGAATATT TGTATGTAAA ATAGTGTGCT GTGCACTCTG TTCACACGAG ATGGAATGCT GGTAACTTAA CTGTCTGGCT CTCCAGGAAT ACCTGTGTTA ACTGTAGTGC ATTTTGCCTT CTTTCCTTCT TAGAGTCACC TCTACTTTCC TGCTACCGCT GCCTGTGAGC TGAAGGGGCT GAACCATACA CTCCTTTTTC TACAACCAGC TTGCATTTTT TCTGCCCACA GTGTCAGTCA ATACTTCATA TTACTCAGTT GATTCTGAGA TGTTACTGTG CTCCTTGCAG GAGGTCAGGC AGTTCTCCAG GCTATTTGTA CCGATTGCCT ACTCCTTGAT CTGTGTCTTT GGCCTCCTGG GGAATATTCT GGTGGTGATC ACCTTTGCTT TTTATAAGAA GGCCAGGTCT ATGACAGACG TCTATCTCTT GAACATGGCC ATTGCAGACA TCCTCTTTGT TCTTACTCTC CCATTCTGGG CAGTGAGTCA TGCCACCGGT GCGTGGGTTT TCAGCAATGC CACGTGCAAG TTGCTAAAAG GCATCTATGC CATCAACTTT AACTGCGGGA TGCTGCTCCT GACTTGCATT AGCATGGACC GGTACATCGC CATTGTACAG GCGACTAAGT CATTCCGGCT CCGATCCAGA ACACTACCGC GCAGCAAAAT CATCTGCCTT GTTGTGTGGG GGCTGTCAGT CATCATCTCC AGCTCAACTT TTGTCTTCAA CCAAAAATAC AACACCCAAG GCAGCGATGT CTGTGAACCC AAGTACCAGA CTGTCTCGGA GCCCATCAGG TGGAAGCTGC TGATGTTGGG GCTTGAGCTA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_004367, NM_031409
Synonyms	BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CD196; CKR-L3; CKRL3; CMKBR6; DCR2; DRY6; GPR29; GPRCY4; STRL22
Summary	This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

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