ACADVL Human Gene Knockout Kit (CRISPR)

CAT#: KN204428

ACADVL - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Rabbit Polyclonal Anti-ACADVL Antibody
    • 50 ug

CNY 4,628.00


ACADVL (Myc-DDK-tagged)-Human acyl-CoA dehydrogenase, very long chain (ACADVL), nuclear gene encoding mitochondrial protein, transcript variant 2
    • 10 ug

CNY 4,712.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol ACADVL
Locus ID 37
Kit Components

KN204428G1, ACADVL gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTGGCCGCGGCGGCGGGTAA

KN204428G2, ACADVL gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTTTCGTTCCTCCCCTCCGT

KN204428-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TAACAGTTCC CTCAAATTGA GAGAGCATCC CTAAATGCAG CCACCACGAG ATGCTTGTTC TAGAGTCTGA AGGCCCACCC CTTCCCTGCC AGCTGACACC CCCAGATCAG TCAGGCACTC ACAACCAATA ACCTTTACCC CATGCCAGGA GCCCCCCAAA ACTGGACAAG GCCCTTGGAA GCCAGCTTTT CCCTGGGGAG GAAAGAGGAA CCCCCCAACA TAGCCCACCT TCCCACATAC ACCAGCAGGA CTCCCTTATG AGCCAGGCTT CTCCTTAAAT AAGTCTCCCT CAAACAGCGC TGATGAACCC CCAAACCCGC CAGGCCCCCC GAACCATGGA ATATCCTAAA TTTTCCATAT CCCTACAGGT ACAAAGGGAT CACTCCAGGG GGCACCTGTG GCCAAATCCT GCATTTTCTC AGATGTGAAC CCCAAAACTA AATTAAGACC CCCAAATACA TCTTACTTCT GATGCTGCCA CTCCAGGGGC GCCAGTCCCA GATAAGGCCT ACTAACCCCT CCCCACAAAC TCATACCCTC CCCCCAGTTT ATAGCCCCCC CATCATGCTT ACCTTGGTTG TCACTATACA GAGACAGTCC AGGATGGGGG GAGGGGTGAG AGGGGAAGGA GGGGGTTGGA GAAGGGAAGG GGAGGGGAGC GTGGGAGGGA GGGGAAGGGG GCCCTAAAAG GGGCTCCCCA GTGGAGGGGA GGGGGCTTGG GGAGCACACC CCGATTCTCA GGAGGGGCGG GGGCACCGGG GGCTGGCAGC CCCGGAGTTC GGGGGCTGGG GCATGAGCTG GGGTGGGGGA GGGGAACTGG ATTTCGGGGA GCCCCGGGGT AGGGGGGGGT CTTGCCAAAC GGCCAGGGGC TAGGGGCCGT GGCGGGGGAG TGGGGTGGGG GGGTTGGAAA CGGCAGCGGC CGAGGGAGCC GTGGAGCCGA AGAGGGAAGG GGAGAGAGGA GGAGAGAGGA AGCAGGGGGA GGGAGGGAGG GAGCTAGGAG CCAGAATGGG GGGGGTGCCT TGGCAGAGTT AACTCCTTCT GTGCTGGCAG GAACCCGGAT AATGGGAAAG GAGATAGAAG CAGAAGCACA GAGGCCCCTG AGGCAAACAT GGAGACCTGG CCAGCCACCA GCGATGACAG CAAAGACGAT GAGGCTGGAT TTCATTTCTT AGAGAAGGAA GCATCTATAG TTGGGCTGGG

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000018, NM_001033859, NM_001270447, NM_001270448
Synonyms ACAD6; LCACD; VLCAD
Summary The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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