TNFRSF1A Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	TNFRSF1A
Locus ID	7132
Kit Components	KN204008G1, TNFRSF1A gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAGCAGCAGGTCAGGCACGG KN204008G2, TNFRSF1A gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTTGTCCCTGGTCTCACCAG KN204008-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GTGTCTGAGA AGTTCATTTA TGTGTCCAAG ACACATTCTT AGCTAAAAAG CTAAGCATTC TGAATTGGAA CCCAGAGAAT TTGACTCCCA GACTCTGGAT CTTTTCACTG CTGTGATCCA TCTGGGAAAG GCTAGTGATG TGGGCAAGGG GCTTATTGCC CCTTGGTGTT TGGTTGGGAG TGGTCGGATT GGTGGGTTGG GGGCACAAGG CAGCCAGATC TGGGACTCCT GTGCTTGTGA CTGGACTACA AAGAGTTAAA GAACGTTGGG CCTCCTCCTC CCGCCTCCTG TGGCCTCCTC CTCCAGCTCT TCCTGTCCCG CTGTTGCAAC ACTGCCTCAC TCTTCCCCTC CCACCTTCTC TCCCCTCCTC TCTGCTTTAA TTTTCTCAGA ATTCTCTGGA CTGAGGCTCC AGTTCTGGCC TTTGGGGTTC AAGATCACTG GGACCAGGCC GTGATCTCTA TGCCCGAGTC TCAACCCTCA ACTGTCACCC CAAGGCACTT GGGACGTCCT GGACAGACCG AGTCCCGGGA AGCCCCAGCA CTGCCGCTGC CACACTGCCC TGAGCCCAAA TGGGGGAGTG AGAGGCCATA GCTGTCTGGC TGGGCGAGGC ACCTTCCGGC TGGCGTGGGC CCTCTCCGGG AGGGGGCCGA GCCTCTCCTG CCCGGGCCTG GTCCTGGCGC CAGCCTCAGG CCTGCAGGTC CTAACCTCAG CCACTGCCAG TGTGGGGTTC CCCATTCATC CGCCTTTTGG AGTAGGGGCT GCGCTGAGGC AGGGGAATGG GAGAAGTTTG AAAGGGAGAG AGTAAAAGGA AGCCCTGGCC CCTGACAGCG GTGGAAGTTT GTGGGCGGCC AAGGGAATGT GGGCAGGAGA TAGGCCCAGG GTGGGGCAGA TTTGGCGGGG AAAAGAAGGG AGTGGGAGTA GGAAGATTAG TGCTCGGGGA GTCCAGACGG TTCTGAATTC TGTCCCTCCG GTCAGCTGGC TGGCCTGGAG GGTGTTGGGC CGTGGGGAGG CGAGGCTGCC TGTGGAACTT GGTGGAGCAC ACCCTGTAGG GCAGGATTTT GGCGGCTGGT GAAGTGGGGG AGTGAGTTGA GGAGTGGGGA TGGGCTGGTG TGGTGGGTTT GGGATGCTCA TGGTGGGAGG TATTTGAGAA TGGGCTGGGA CACTGGATGG GGCAGGGCAA CCCAGTGGAC AGTGTCCCCA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001065, NM_001346091, NM_001346092, NR_144351
Synonyms	CD120a; FPF; MS5; p55; p55-R; p60; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR1-d2; TNFR55
Summary	This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016]

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