FKBP10 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	FKBP10
Locus ID	60681
Kit Components	KN203676G1, FKBP10 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TACTGGTGGTGCAGGCCGTG KN203676G2, FKBP10 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCTACTGGTGGTGCAGGCCG KN203676-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet ACAAAGTCCT TCTTTCAGTC CAGCTTTTGC GGGACCAAGC TGCTCTTCCC TGGAGATGCC TTCCCTTGGA GTACAGCTGG GGTCACGGAT GAGCACGGAG ATGCCTCTCA CACTCCGCGG CTTCACCACC CACCTGGAGG GGACTAGAAT TCGAAGTGCC CGCGAAAGAA GCAAGGTTCA TCTCCGCCCG GCTTTAGGAC CCGGGGGCTC CGGAAAAAGT CATTGAACTT TTTTTTTTTT TTTTCCTTTG GTGTCCACTC AGAGTTCTCA TCTTTCCCGA GCCTCTCTCC CTGGCCAGGC CCCAGGTCTC GCAGCCAGGG ATGGAGATGG GGGGAGGGGG AACCTAGAGT TCTTTGTAGT GCCTCCCTCA GACTCTAACA CACTCAGCCT GGCCCCCTCC TCCTATTGCA ACCCCCTCCC CCGCTCCTCC CGGCCAGGCC AGCTCAGTCT TCCCAGCCCC CATTCCACGT GGACCAGCCA GGGCGGGGGT AGGGAAAGAG GACAGGAAGA GGGGGAGCCA GTTCTGGGAG GCGGGGGGAA GGAGGTTGGT GGCGACTCCC TCGCTCGCCC TCACTGCCGG CGGTCCCAAC TCCAGGCACC CGAGAGGTAC CACATCCCCA GGGCCTGTCC CCGGGAAGTG CAGATGGGGG ATTTTGTGCG CTACCACTAC AACGGCACTT TTGAAGATGG CAAGAAGTTT GATTCAAGGT AACCCCGGTT GGGCGCCCCC GGATTCACCA CTCCGTCCCC TGAACCCGGG GTCCTGTTTC CTTGTTGCCT CTTTTAAGCT CTGCATCCCA ATACTCTAAC CCTAGACAGC ACCCCTGGGG CCTCCCAGAC ACCCATCTCC CCAAAGATAC CCTCCTGCCC CCACTTCCAA ACCCTCACTT CTCAAAGGAT CCCATTTCTT TCTCACAAGC TCTCCACATC CCGGAAAGGA CTCGATAATC AAGGTGAACT GCCTTGTGTA GTTACCAGGG CTGTATGTAA TGACCTGATG GAGAGGGGTG TTCTGATCTA CAGTGTTGGG GGGAAGGGGG TGGTCCCTGC TGCTTAAGAA CCCTGGGATG GTCTATCAGC CTGGACCCCG TACCTTTCCA CCCACCCTGA CTCCCCTTCT GGTTCCACTG TTTTCAGTGC CTTTGTGGTC AGAGAAGAGG GAATAGAACT TTTTTTTGGA GGGGGTGGGA GTTTTCAGCG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_021939
Synonyms	BRKS1; FKBP65; hFKBP65; OI6; OI11; PPIASE
Summary	The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.[provided by RefSeq, Nov 2009]

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