RGS10 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	RGS10
Locus ID	6001
Kit Components	KN203488G1, RGS10 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGCCGTCAGGTAAGCGGCTT KN203488G2, RGS10 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCTCAGCCGGCTCACGGCG KN203488-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GAGGCCTAGG CTTCCCAGCC TGTGACCCGG GAGGGGACCC CGCCCGCCAC CTGCGAAGGC TCGCTGAGAG CCCCGAAGCG CGGTGCGCCC GCGGCGCCGT GCCAGTTTCT CCGGGGCTAC AGGGAGAAGG CGGGAGACCG CGATGGGCCC GGGAGCTTGA AGCTGGGCGG GGCGGGCCGG GGCGGCATCT CAGGCGAGGG CCAGGGACCC CGGGACGCGC TAACCGCCTC CGCGGGGCTA CTAAGTTCCC GGCGGCGCGG GCTCCCTCTG GGCCAGTGCA CCTTATCCCA GGCGCGGGGC ACACTCTGGG CGATCGGGGG TCACCCGGGG CTGCCCTGCC CTGCCCCGCC CGGGCACCCC GGAGCGGAAC CGCGAGTCCT CACCAGCGGG GGCGGGCAGG GCGCACTGAG CGCGCCACGC GGCGCCTGGC TAGGAGGAGG GCGGCGGGCG GGGACCTAGA GCTCCGGGGC GGTGCTGAGC CCGCTCCTCC TCCTTGCCCT CCTCCTCCTC CTCCTCGCCC TCCTCCTCCT CCTCGCCTTC CTCCGGCTCA GCCGCCGCGC CGCCGGGCTG CTCCTTCTTC CTCCTCGGGC GCCCGCGGCG CCCGGCCTCC TGCCCTCCCT CTTGTGCTCG GGCGCGGCTC GGTGGTACCT CCCAGACTGG AGCCCCGGCC GACCGCACCA CCTCTCCCCG CTGGGTACCG CACCTTCGCG GTCCCAGCCG CGACGGGAGC GCAGAGCTCC TCCGGGAGCC CCGGGGAGGA AGTTTGGCTG AAAGAGGAAC AGCCGCAGTT TTCTTTTTAG TGATGGTCTG GGAGAAAAAG GAGCGCCCCA AATCTCCAAA CTTTGGAGGT GACATGGGGC TCCAGATTCC GAAAGCGGAA CGGCGCGCGG GCTTCTCCCT CCGTGACGCT CTCCGCCTCC GCTGCGGGCG TGGGTGGAAG GATGCCGAGC GCCCGGGGGA GCACGGCTGG ACCCGGGCAT CCGACTGGGC CCCTGGGCCT GGGGCGTCGG GGGCCTTCGC TTCTGCCGCG AGGAGCCAAA AGGTGGGATC GCGAGGGTGG CGGAACCCCA GGATGGAGAA GGGCACTTCT GCGGTCCGAG CCAGCGAAGT TCTGACGTTA CGAAGGATTC GCCTTGGCCG TCACTTTGGG CCAGCCTGAG CCACGATCTC GGGCAACTTG ATAGGCCAAA GACCCCACGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001005339, NM_002925
Synonyms	OTTHUMP00000020597; OTTHUMP00000069158; regulator of G-protein signaling 10; regulator of G-protein signalling 10
Summary	Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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