ERCC8 Human Gene Knockout Kit (CRISPR)

CAT#: KN203124

ERCC8 - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


ERCC8 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


ERCC8 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 8 (ERCC8), transcript variant 3
    • 10 ug

CNY 2,400.00
CNY 3,705.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol ERCC8
Locus ID 1161
Kit Components

KN203124G1, ERCC8 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTCCGCACGCCAAACGGGTT

KN203124G2, ERCC8 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGAGAGCAGAGTCAACACGG

KN203124-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

GGACCGCGGA CGCACGAGGC CGGGCCAAGG CGCCCAGAGC CGGGCCGGGT AGGGCTCCGA TGCCAGAGTG AAGGGGTCGA CAGGGGAGAA CGAACTCGGG AAATTCTCTC CGGTGACACC CTAAGTGTCG CATGATGCGT TAGTTGACTA GGGGCTCTCA CTTTTCCCTC CTTTACTGAC CTCGCAATCA CTGACCACGC AATCGCTGCC CACGCCGCCA CCTCACCTCT CCAGTTCTTG TACTGCGGGA TGTAGTAGTA TTTGTTCCCG AATTGGTCCG TGCCCACGTG CTCCTTCACT TCCCTTGACA GCGATCTCCA CAAGGCGCGG AACAAATCCT GAGACCAACC CATGCCGTCC ACCCAGTTTC CAGCGCTGCC AGCAGCGGGA CCCGCAGTAG GGGTAATGCT CCAGCCGCCG ACCCAGCCCA CCGAGGCGGA GAAGTCAGGT CGGCCTCCAT CCTGGAGCCC TTCTAGTAAC GGCGCTCACC ATTGGCCGGA TATATCACCG ACTGACCAAT GGGAGACTCT GCTGTTCCAG TCCCGCCCCT GAGCTCTGTC ATGGCGACGT CCAGTGCTCC AGCCGGTGTG AGGACACGAT AGGTAAAGAA AACCTTACTT TTTGGCTAAC AGGCCAGTTA GAATCTGGAC CAATGACTGT AAGCTTTGCC CCACACTTTC CCGTCTCATC GCTCTCCTGC CACCAAACTA TTATTTGCCC TCTAATGCTG TGTGGCTTGC TAAAATAGGA TCCCTTTTAT TTACTGGTAA CAACTTGCAG GCGGGGGGCG GCGGGGGTGG GGGGGGTTCC CCGGGGGTGG GGGGGGGGTT CCCCCGCGGG CTCAGGTGAA GACACTATAA ACCTAAATAA GAATACTTCG TTGTACTGTA TTCCTTCTCT CTTAAGTTAC TCCAAAGAAT AACTGCCCTC CACGGACTCG TTCATTCATT CAGTCTGCAT ATTTAGTGAA TTCCTACAGT GTGCCAGGCA GCCTATTAAA GGCAGAGGAC ACAGGCCAGG AATTGAACAA AGTTCAGAAT GACTAGAGCG GAGAATTGGG GTAGGGACCA TTGACAAGAG ATGAAGTAGA CAGAAACTAG ATGAGGAAGA GCCTTGTGTA CTTGAGTAGA GGTGTGAAGA TGAGAATAAG GTAACAGCTA TTGCATTTTG TTTTCTGCTT GGAGAATGGG TTGCAACTGA

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000082, NM_001007233, NM_001007234, NM_001290285
Synonyms CKN1; CSA; UVSS2
Summary This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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