S100A3 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	S100A3
Locus ID	6274
Kit Components	KN202117G1, S100A3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTCCTGGAAGGTGCACACGA KN202117G2, S100A3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGAATACGCAGGGCGCTGTG KN202117-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AGGATCCCAC AAGTTTTTCT TCTGGGTGCA GCTTCCTCGA GTTCAGGGCA GGAGGCAGGG TGAGGGCAGT GGTGGTGAGG CTGTGGGGGC GCCTCTCAGA CCTGCCCCTC CCTGCTAAGG CCTAGCTTCT CCAAGGGCTG AGGGCAGAGA AGCACAGGGC CAGGCTCTCC TTGTGCAAGT TCCTGCAGGG AGGGAGGCTT CTAGGGCATC TCATGGGCTG GGGCAGACTG GGACCGAGCC ATCCCTGGGT GTTGGGGATA AGAAGAGGTG GCAGATAATC TGAGAGTGCT TAAGGAGCCC CACGGTGGGG TGTGTGGGTG GTGGGGTAGG ATGCTGTCTC TCTGAGGAGT TTACAGCGAT GTTGGGGCAA TGAGACCTCT ACATGGGGAA ATAACAACAG GACAGCAGGG GGATAAATTA GGAGGGGCTG GTGTGGTCTG GGAGGGCTAC TCAGAGGAGG GGGAGACTGC GGGGCCTGAA GCCTGATGGA GGTTAAGATG GGTCTGGGAA CAGTCTCTGA GAAACACAGA GGGCAGAGGT GAGCTGGGGG GCTGGCCTGG AACTCTGCTG TGAGCCACAC CCTTGTTCTG TGACAGTGAG TCTGCCAGGC GGAGCTCAAG GAGCTGCTGC AGAAGGAGCT GGCCACCTGG ACCCCGGTGA GCAGTCAGAG TGTCCGTCCC CTCCTCCTCC GGGGGGAGGC ACTGCCTAGA CTCTGCAAAG AGAGACCTGG GGACAACAAG CATGAGCACA GGACAGCAAG GGAGGGGGCT GGATTGTGAG GAGGGAGTGG GAGGGGGAGA ACTGACACAG CAGAAACCTC AGCTTCCTCA TCTGTAAAAT GGGATGACAA TCATCCCTGC TGCCTTGCAG AGCTGTTGAG AGGACTGAGT GAGAGCACCA TGAAGAATGC CTAACACTTG GTAGGCACTC AGTAGCAAAT AGTAGGTGCT CGATTAATAA TCTGAGAGGA CAGATTTGGG AGGGGATTGC AACGAGTGCA TTATCAGAGC AGGGAGGTTG AAGTCGGCCC TCAGAAGGGT GTAGTTTGGG GTGGGGTGGG TCCACTTGGG AGAGGAAGGA GGGAGGTGGC GTGTGAGGCT GTTCTGCCCT CCTCATCCTA CTCCCTTACC TCTCCCAACC CTTCCCTTGC ACAGACTGAG TTTCGGGAAT GTGACTACAA CAAATTCATG AGTGTTCTGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_002960
Synonyms	S100E
Summary	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has the highest content of cysteines of all S100 proteins, has a high affinity for Zinc, and is highly expressed in human hair cuticle. The precise function of this protein is unknown. [provided by RefSeq, Jul 2008]

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