MGAT1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA	Luciferase-Puro
Symbol	MGAT1
Locus ID	4245
Kit Components	KN200717G1, MGAT1 gRNA vector 1 in pCas-Guide CRISPR vector KN200717G2, MGAT1 gRNA vector 2 in pCas-Guide CRISPR vector KN200717LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. Homologous arm and Luciferase-Puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet TGTTAAATAA CCCTGGAGAT GATGGGCAAC TTCGTTTTGC TCCTGACATT CGTGGGGTGC CTCTGGTGCT TCCCTGTTGG TAAGGGGTTA ACTGTAGCCC TGAGGTGGGA CATTTGATTT TAAAAATCAG TCATCTTGGG GCGCTTAGGT TAGAGGAATG GTAGGCAGAT GCTGTCACTC CTTGCCCCTC CCCTCCTCCT TCCCACCTGG AGGGGAAATG AAATCTGACA GGTAGAAAGA GGGGAGTTGG GGTTCTTTTT CTCTCTCCCT CCACCAGCAT CACTCTCTGC CTCTCCCTCA AAAATACGTT CCTGGGTCAG GATATATGTT GACTCCCTAG AGAGCTCTGG AGTCAACCTC CTGGCCTTCC TCCACCCTCA CTCTTGGCCT TTTCCTGCCC CCATTTCCTC TACCTGTGGG GCATGGAGCC ACGAGCCTTT GTGTGACGGT TTGCTTTCTC TCTCCTGTCT TTAGGTGCAT GGCTGCCTCC TAATCCCATA GTCCAGAGGA GGCATCCCTA GGACTGCGGG CAAGGGAGCC GGGCAAGCCC AGGGCAGCCT TGAACCGTCC CCTGGCCTGC CCTCCCCGGT GGGGGCCAGG TCGATGGCGA CCCCGCCAGC CTCACCCGGG AAGTGATTCG CCTGGCCCAA GACGCCGAGG TGGAGCTGGA GCGGCAGCGT GGGCTGCTGC AGCAGATCGG GGATGCCCTG TCGAGCCAGC GGGGGAGGGT GCCCACCGCG GCCCCTCCCG CCCAGCCGCG TGTGCCTGTG ACCCCCGCGC CGGCGGTGAT TCCCATCCTG GTCATCGCCT GTGACCGCAG CACTGTTCGG CGCTGCCTGG ACAAGCTGCT GCATTATCGG CCCTCGGCTG AGCTCTTCCC CATCATCGTT AGCCAGGACT GCGGGCACGA GGAGACGGCC CAGGCCATCG CCTCCTACGG CAGCGCGGTC ACGCACATCC GGCAGCCCGA CCTGAGCAGC ATTGCGGTGC CGCCGGACCA CCGCAAGTTC CAGGGCTACT ACAAGATCGC GCGCCACTAC CGCTGGGCGC TGGGCCAGGT CTTCCGGCAG TTTCGCTTCC CCGCGGCCGT GGTGGTGGAG GATGACCTGG AGGTGGCCCC GGACTTCTTC GAGTACTTTC GGGCCACCTA TCCGCTGCTG AAGGCCGACC CCTCCCTGTG GTGCGTCTCG GCCTGGAATG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001114617, NM_001114618, NM_001114619, NM_001114620, NM_002406, NM_001364390, NR_157150, NR_157151, NM_001364379, NM_001364380, NM_001364384, NM_001364386, NM_001364388, NM_001364391, NM_001364392, NM_001364393, NM_001364394, NM_001364377, NM_001364381, NM_001364382, NM_001364383, NM_001364385, NM_001364387, NM_001364389, NM_001364395, NR_157152, NR_157153, NR_157154
Synonyms	GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I; MGAT
Summary	There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

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