Mouse Gen1 activation kit by CRISPRa
CAT#: GA212925
Gen1 CRISPRa kit - CRISPR gene activation of mouse GEN1, Holliday junction 5' flap endonuclease
CNY 12,255.00
Specifications
Product Data | |
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Symbol | Gen1 |
Locus ID | 209334 |
Kit Components | GA212925G1, Gen1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA212925G2, Gen1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA212925G3, Gen1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer vector, SKU GE100056 1 CRISPRa scramble vector, SKU GE100077 |
Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc. |
Reference Data | |
RefSeq | NM_177331 |
Synonyms | 5830483C08Rik |
Summary | Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates.[UniProtKB/Swiss-Prot Function] |
Documents
Resources
基因表达相关资源 |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
KN506409 | Gen1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. |
CNY 8,680.00 |