Human FAM21C (WASHC2C) activation kit by CRISPRa

Specifications

Product Data
Format	3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Symbol	WASHC2C
Locus ID	253725
Kit Components	GA116754G1, FAM21C gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA116754G2, FAM21C gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA116754G3, FAM21C gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer vector, SKU GE100056 1 CRISPRa scramble vector, SKU GE100077
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
Reference Data
RefSeq	NM_001169106, NM_001169107, NM_015262, NM_001330074, NM_001367394, NM_001367395, NM_001367396, NM_001367399, NM_001367403, NM_001367407, NM_001367409, NM_001367412, NM_001367413, NM_001367415, NR_159966, NM_001367393, NM_001367397, NM_001367398, NM_001367400, NM_001367401, NM_001367402, NM_001367404, NM_001367405, NM_001367406, NM_001367408, NM_001367410, NM_001367411, NM_001367414, NM_001367416
Synonyms	FAM21A; VPEF
Summary	Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes inhibits WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization and is involved in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission (PubMed:19922874, PubMed:20498093, PubMed:22513087, PubMed:23331060). Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35 (PubMed:24980502). Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L (PubMed:25355947, PubMed:28892079).[UniProtKB/Swiss-Prot Function]

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