MASTL (NM_032844) Human Mutant ORF Clone

Specifications

Product Data
Product Name	MASTL (NM_032844) Human Mutant ORF Clone
Mutation Description	E167D
Affected Codon#	167
Affected NT#	501
Nucleotide Mutation	MASTL Mutant (E167D), Myc-DDK-tagged ORF clone of Homo sapiens microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2 as transfection-ready DNA
Effect	Thromboyopeni
Synonyms	GREATWALL; GW; GWL; MAST-L; THC2
Vector	pCMV6-Entry
Tag	Myc-DDK
ACCN	NM_032844
ORF Size	2634 bp
Sequence Data	ORF Nucleotide Sequence (showhide) >RC403509 representing NM_032844 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCACCGCGGGAAGCAAGAAGGAGCCTGGAGGAGGCGCGGCGACTGAGGAGGGCGTGAATAGGA TCGCAGTGCCAAAACCGCCCTCCATTGAGGAATTCAGCATAGTGAAGCCCATTAGCCGGGGCGCCTTCGG GAAAGTGTATCTGGGGCAGAAAGGCGGCAAATTGTATGCAGTAAAGGTTGTTAAAAAAGCAGACATGATC AACAAAAATATGACTCATCAGGTCCAAGCTGAGAGAGATGCACTGGCACTAAGCAAAAGCCCATTCATTG TCCATTTGTATTATTCACTGCAGTCTGCAAACAATGTCTACTTGGTAATGGAATATCTTATTGGGGGAGA TGTCAAGTCTCTCCTACATATATATGGTTATTTTGATGAAGAGATGGCTGTGAAATATATTTCTGAAGTA GCACTGGCTCTAGACTACCTTCACAGACATGGAATCATCCACAGGGACTTGAAACCGGACAATATGCTTA TTTCTAATGACGGTCATATTAAACTGACGGATTTTGGCCTTTCAAAAGTTACTTTGAATAGAGATATTAA TATGATGGATATCCTTACAACACCATCAATGGCAAAACCTAGACAAGATTATTCAAGAACCCCAGGACAA GTGTTATCGCTTATCAGCTCGTTGGGATTTAACACACCAATTGCAGAAAAAAATCAAGACCCTGCAAACA TCCTTTCAGCCTGTCTGTCTGAAACATCACAGCTTTCTCAAGGACTCGTATGCCCTATGTCTGTAGATCA AAAGGACACTACGCCTTATTCTAGCAAATTACTAAAATCATGTCTTGAAACAGTTGCCTCCAACCCAGGA ATGCCTGTGAAGTGTCTAACTTCTAATTTACTCCAGTCTAGGAAAAGGCTGGCCACATCCAGTGCCAGTA GTCAATCCCACACCTTCATATCCAGTGTGGAATCAGAATGCCACAGCAGTCCCAAATGGGAAAAAGATTG CCAGGAAAGTGATGAAGCATTGGGCCCAACAATGATGAGTTGGAATGCAGTTGAAAAGTTATGCGCAAAA TCTGCAAATGCCATTGAGACGAAAGGTTTCAATAAAAAGGATCTGGAGTTAGCTCTTTCTCCCATTCATA ACAGCAGTGCCCTTCCCACCACTGGACGCTCTTGTGTAAACCTTGCTAAAAAATGCTTCTCTGGGGAAGT TTCTTGGGAAGCAGTAGAACTGGATGTAAATAATATAAATATGGACACTGACACAAGTCAGTTAGGTTTC CATCAGTCAAATCAGTGGGCTGTGGATTCTGGTGGGATATCTGAAGAGCACCTTGGGAAAAGAAGTTTAA AAAGAAATTTTGAGTTGGTTGACTCCAGTCCTTGTAAAAAAATTATACAGAATAAAAAAACTTGTGTAGA GTATAAGCATAACGAAATGACAAATTGTTATACAAATCAAAATACAGGCTTAACAGTTGAAGTGCAGGAC CTTAAGCTATCAGTGCACAAAAGTCAACAAAATGACTGTGCTAATAAGGAGAACATTGTCAATTCTTTTA CTGATAAACAACAAACACCAGAAAAATTACCTATACCAATGATAGCAAAAAACCTTATGTGTGAACTCGA TGAAGACTGTGAAAAGAATAGTAAGAGGGACTACTTAAGTTCTAGTTTTCTATGTTCTGATGATGATAGA GCTTCTAAAAATATTTCTATGAACTCTGATTCATCTTTTCCTGGAATTTCTATAATGGAAAGTCCATTAG AAAGTCAGCCCTTAGATTCAGATAGAAGCATCAAAGAATCCTCTTTTGAAGAATCAAATATTGAAGATCC ACTTATTGTAACACCAGATTGCCAAGAAAAGACCTCACCAAAAGGTGTCGAGAACCCTGCTGTACAAGAG AGTAACCAAAAAATGTTAGGTCCTCCTTTGGAGGTGCTGAAAACGTTAGCCTCTAAAAGAAATGCTGTTG CTTTTCGAAGTTTTAACAGTCATATTAATGCATCCAATAACTCAGAACCATCCAGAATGAACATGACTTC TTTAGATGCAATGGATATTTCGTGTGCCTACAGTGGTTCATATCCCATGGCTATAACCCCTACTCAAAAA AGAAGATCCTGTATGCCACATCAGACCCCAAATCAGATCAAGTCGGGAACTCCATACCGAACTCCGAAGA GTGTGAGAAGAGGGGTGGCCCCCGTTGATGATGGGCGAATTCTAGGAACCCCAGACTACCTTGCACCTGA GCTGTTACTAGGCAGGGCCCATGGTCCTGCGGTAGACTGGTGGGCACTTGGAGTTTGCTTGTTTGAATTT CTAACAGGAATTCCCCCTTTCAATGATGAAACACCACAACAAGTATTCCAGAATATTCTGAAAAGAGATA TCCCTTGGCCAGAAGGTGAAGAAAAGTTATCTGATAATGCTCAAAGTGCAGTAGAAATACTTTTAACCAT TGATGATACAAAGAGAGCTGGAATGAAAGAGCTAAAACGTCATCCTCTCTTCAGTGATGTGGACTGGGAA AATCTGCAGCATCAGACTATGCCTTTCATCCCCCAGCCAGATGATGAAACAGATACCTCCTATTTTGAAG CCAGGAATACTGCTCAGCACCTGACTGTATCTGGATTTAGTCTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGA TAAGGTTTAA Protein Sequence (showhide) >RC403509 representing NM_032844 Red=Cloning site Green=Tags(s) MDPTAGSKKEPGGGAATEEGVNRIAVPKPPSIEEFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMI NKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEV ALALDYLHRHGIIHRDLKPDNMLISNDGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQ VLSLISSLGFNTPIAEKNQDPANILSACLSETSQLSQGLVCPMSVDQKDTTPYSSKLLKSCLETVASNPG MPVKCLTSNLLQSRKRLATSSASSQSHTFISSVESECHSSPKWEKDCQESDEALGPTMMSWNAVEKLCAK SANAIETKGFNKKDLELALSPIHNSSALPTTGRSCVNLAKKCFSGEVSWEAVELDVNNINMDTDTSQLGF HQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQNTGLTVEVQD LKLSVHKSQQNDCANKENIVNSFTDKQQTPEKLPIPMIAKNLMCELDEDCEKNSKRDYLSSSFLCSDDDR ASKNISMNSDSSFPGISIMESPLESQPLDSDRSIKESSFEESNIEDPLIVTPDCQEKTSPKGVENPAVQE SNQKMLGPPLEVLKTLASKRNAVAFRSFNSHINASNNSEPSRMNMTSLDAMDISCAYSGSYPMAITPTQK RRSCMPHQTPNQIKSGTPYRTPKSVRRGVAPVDDGRILGTPDYLAPELLLGRAHGPAVDWWALGVCLFEF LTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWE NLQHQTMPFIPQPDDETDTSYFEARNTAQHLTVSGFSL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq	NP_116233
RefSeq Size	2634 bp
RefSeq ORF	2637 bp
Locus ID	84930
Domains	pkinase, TyrKc, S_TKc
Protein Families	Druggable Genome, Protein Kinase
MW	96.6 kDa
Gene Summary	This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]

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