PKC eta (PRKCH) (NM_006255) Human Mutant ORF Clone

Specifications

Product Data
Product Name	PKC eta (PRKCH) (NM_006255) Human Mutant ORF Clone
Mutation Description	v374I
Affected Codon#	374
Affected NT#	1120
Nucleotide Mutation	PRKCH Mutant (v374I), Myc-DDK-tagged ORF clone of Homo sapiens protein kinase C, eta (PRKCH) as transfection-ready DNA
Effect	Cerebral infarction, association with
Synonyms	nPKC-eta; PKC-L; PKCL; PRKCL
Vector	pCMV6-Entry
Tag	Myc-DDK
ACCN	NM_006255
ORF Size	2049 bp
Sequence Data	ORF Nucleotide Sequence (showhide) >RC402800 representing NM_006255 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCTGGCACCATGAAGTTCAATGGCTATTTGAGGGTCCGCATCGGTGAGGCAGTGGGGCTGCAGC CCACCCGCTGGTCCCTGCGCCACTCGCTCTTCAAGAAGGGCCACCAGCTGCTGGACCCCTATCTGACGGT GAGCGTGGACCAGGTGCGCGTGGGCCAGACCAGCACCAAGCAGAAGACCAACAAACCCACGTACAACGAG GAGTTTTGCGCTAACGTCACCGACGGCGGCCACCTCGAGTTGGCCGTCTTCCACGAGACGCCCCTGGGCT ACGACCACTTCGTGGCCAACTGCACCCTGCAGTTCCAGGAGCTGCTGCGCACGACCGGCGCCTCGGACAC CTTCGAGGGTTGGGTGGATCTCGAGCCAGAGGGGAAAGTATTTGTGGTAATAACCCTTACCGGGAGTTTC ACTGAAGCTACTCTCCAGAGAGACCGGATCTTCAAACATTTTACCAGGAAGCGCCAAAGGGCTATGCGAA GGCGAGTCCACCAGATCAATGGACACAAGTTCATGGCCACGTATCTGAGGCAGCCCACCTACTGCTCTCA CTGCAGGGAGTTTATCTGGGGAGTGTTTGGGAAACAGGGTTATCAGTGCCAAGTGTGCACCTGTGTCGTC CATAAACGCTGCCATCATCTAATTGTTACAGCCTGTACTTGCCAAAACAATATTAACAAAGTGGATTCAA AGATTGCAGAACAGAGGTTCGGGATCAACATCCCACACAAGTTCAGCATCCACAACTACAAAGTGCCAAC ATTCTGCGATCACTGTGGCTCACTGCTCTGGGGAATAATGCGACAAGGACTTCAGTGTAAAATATGTAAA ATGAATGTGCATATTCGATGTCAAGCGAACGTGGCCCCTAACTGTGGGGTAAATGCGGTGGAACTTGCCA AGACCCTGGCAGGGATGGGTCTCCAACCCGGAAATATTTCTCCAACCTCGAAACTCGTTTCCAGATCGAC CCTAAGACGACAGGGAAAGGAGAGCAGCAAAGAAGGAAATGGGATTGGGGTTAATTCTTCCAACCGACTT GGTATCGACAACTTTGAGTTCATCCGAGTGTTGGGGAAGGGGAGTTTTGGGAAGGTGATGCTTGCAAGAA TAAAAGAAACAGGAGACCTCTATGCTGTGAAGGTGCTGAAGAAGGACGTGATTCTGCAGGATGATGATGT GGAATGCACCATGACCGAGAAAAGGATCCTGTCTCTGGCCCGCAATCACCCCTTCCTCACTCAGTTGTTC TGCTGCTTTCAGACCCCCGATCGTCTGTTTTTTGTGATGGAGTTTGTGAATGGGGGTGACTTGATGTTCC ACATTCAGAAGTCTCGTCGTTTTGATGAAGCACGAGCTCGCTTCTATGCTGCAGAAATCATTTCGGCTCT CATGTTCCTCCATGATAAAGGAATCATCTATAGAGATCTGAAACTGGACAATGTCCTGTTGGACCACGAG GGTCACTGTAAACTGGCAGACTTCGGAATGTGCAAGGAGGGGATTTGCAATGGTGTCACCACGGCCACAT TCTGTGGCACGCCAGACTATATCGCTCCAGAGATCCTCCAGGAAATGCTGTACGGGCCTGCAGTAGACTG GTGGGCAATGGGCGTGTTGCTCTATGAGATGCTCTGTGGTCACGCGCCTTTTGAGGCAGAGAACGAAGAT GACCTCTTTGAGGCCATACTGAATGATGAGGTGGTCTACCCTACCTGGCTCCATGAAGATGCCACAGGGA TCCTAAAATCTTTCATGACCAAGAACCCCACCATGCGCTTGGGCAGCCTGACTCAGGGAGGCGAGCACGC CATCTTGAGACATCCTTTTTTTAAGGAAATCGACTGGGCCCAGCTGAACCATCGCCAAATAGAACCGCCT TTCAGACCCAGAATCAAATCCCGAGAAGATGTCAGTAATTTTGACCCTGACTTCATAAAGGAAGAGCCAG TTTTAACTCCAATTGATGAGGGACATCTTCCAATGATTAACCAGGATGAGTTTAGAAACTTTTCCTATGT GTCTCCAGAATTGCAACCA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGA TAAGGTTTAA Protein Sequence (showhide) >RC402800 representing NM_006255 Red=Cloning site Green=Tags(s) MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNE EFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSF TEATLQRDRIFKHFTRKRQRAMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVV HKRCHHLIVTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQCKICK MNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKESSKEGNGIGVNSSNRL GIDNFEFIRVLGKGSFGKVMLARIKETGDLYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLF CCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHE GHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENED DLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNHRQIEPP FRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq	NP_006246
RefSeq Size	2049 bp
RefSeq ORF	2052 bp
Locus ID	5583
Domains	C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Tight junction, Vascular smooth muscle contraction
MW	75.1 kDa
Gene Summary	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]

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