KRAS (NM_004985) Human Mutant ORF Clone
CAT#: RC400104
- TrueORF®
KRAS mutant (G12D), Myc-DDK-tagged ORF clone of Homo sapiens v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b as transfection-ready DNA
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
货期*
现货
规格
Cited in 7 publications. |
Product images
Specifications
Product Data | |
Product Name | KRAS (NM_004985) Human Mutant ORF Clone |
Mutation Description | G12D |
Affected Codon# | 12 |
Affected NT# | c.35 |
Nucleotide Mutation | KRAS mutant (G12D), Myc-DDK-tagged ORF clone of Homo sapiens v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b as transfection-ready DNA |
Effect | Missense |
Synonyms | C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2 |
Vector | pCMV6-Entry |
Tag | Myc-DDK |
ACCN | NM_004985 |
ORF Size | 564 bp |
Sequence Data |
>RC400104 representing NM_004985
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAA TTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGGATTCCTACAGGAAGCAAGTAGTAATTGA TGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAG TACATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTC ACCATTATAGAGAACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAA ATGTGATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAATTCCT TTTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTATACATTAGTTCGAGAAATTC GAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAAAGAAGAAAAAGAAGTCAAAGACAAAGTGTGTAAT TATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC400104 representing NM_004985
Red=Cloning site Green=Tags(s) MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQ YMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIP FIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NP_004976 |
RefSeq Size | 5312 bp |
RefSeq ORF | 567 bp |
Locus ID | 3845 |
Domains | ras, RAS, RHO, RAB |
Protein Families | Druggable Genome |
Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
Gene Summary | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
Citations (7)
The use of this cDNA Clones has been cited in the following citations: |
---|
High level of ANO1 promotes pancreatic cancer growth in concert with oncogenic KRAS.
,null,
Molecular biology reports
,PubMed ID 36715788
[KRAS]
|
Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method
,null,
Molecular Therapy. Nucleic Acids
,PubMed ID 35474735
[KRAS]
|
The Modulatory Role of MicroRNA-873 in the Progression of KRAS-Driven Cancers
,Mokhlis, HA;Bayraktar, R;Kabil, NN;Caner, A;Kahraman, N;Rodriguez-Aguayo, C;Zambalde, EP;Sheng, J;Karagoz, K;Kanlikilicer, P;Abdel Aziz, AAH;Abdelghany, TM;Ashour, AA;Wong, S;Gatza, ML;Calin, GA;Lopez-Berestein, G;Ozpolat, B;,
Mol Ther Nucleic Acids
,PubMed ID 30654191
[KRAS]
|
KRAS G12D or G12V mutation in human brain arteriovenous malformations
,Oka, M;Kushamae, M;Aoki, T;Yamaguchi, T;Kitazato, K;Abekura, Y;Kawamata, T;Mizutani, T;Miyamoto, S;Takagi, Y;,
World Neurosurg
,PubMed ID 30902772
[KRAS]
|
Mutant KRAS Upregulates BCL-XL via STAT3 to Confer Apoptosis Resistance that is Reversed by BIM Induction and BCL-XL Antagonism
,Zaanan, A;Okamoto, K;Kawakami, H;Khazaie, K;Huang, S;Sinicrope, FA;,
J. Biol. Chem.
,PubMed ID 26245900
[KRAS]
|
Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an independent prognostic marker in wild-type KRAS metastatic colorectal cancer after colorectal liver metastasectomy
,Chen, KF;Yen, CC;Lin, JK;Chen, WS;Yang, SH;Jiang, JK;Lan, YT;Lin, CC;Yu, HC;Hsu, HM;Lin, WL;Teng, HW;,
BMC Cancer
,PubMed ID 25896895
[KRAS]
|
Temsirolimus enhances the efficacy of cetuximab in colon cancer through a CIP2A-dependent mechanism.
,null,
Journal of cancer research and clinical oncology
,PubMed ID 24493623
[KRAS]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
You may also need
Customer
Reviews
Loading...