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Upf1 (NM_030680) Mouse Tagged ORF Clone Lentiviral Particle
CAT＃: MR211651L4V
- LentiORF®
-
Lenti ORF particles, Upf1 (GFP-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2, 200ul, >10^7 TU/mL
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CNY 18,335.00
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Specifications
| Product Data | |
| Product Name | Upf1 (NM_030680) Mouse Tagged ORF Clone Lentiviral Particle |
| Synonyms | B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp |
| Vector | pLenti-C-mGFP-P2A-Puro |
| ACCN | NM_030680 |
| ORF Size | 3339 bp |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR211651).
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| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Reference Data | |
| RefSeq | NM_030680.2 |
| RefSeq Size | 4598 bp |
| RefSeq ORF | 3342 bp |
| Locus ID | 19704 |
| Gene Summary | RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability.[UniProtKB/Swiss-Prot Function] |
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