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Upf1 (NM_030680) Mouse Tagged ORF Clone
CAT＃: MR211651
- TrueORF®
Upf1 (Myc-DDK-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2
ORF Plasmid: tGFP
"NM_030680" in other vectors (4)
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CNY 11,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211651 representing NM_030680
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTGGAGGCGTACGGCCCCAGCTCGCAAACACTCACCTTCTTGGACACTGAGGAGGCCGAGCTGC TCGGCGCCGACACCCAGGGCTCCGAGTTCGAATTCACCGACTTCACCCTTCCCAGCCAGACGCAGACGCC CCCCGGCGGCCCCGGCGGCGCGGGAGGCCCGGGCGGAGCGGGCGCAGGCGGCGCGGCCGGCCAGCTCGAC GCACAAGTTGGACCAGAGGGCATCTTGCAAAATGGGGCTGTGGATGACAGTGTGGCCAAGACCAGCCAGC TGCTAGCTGAGCTGAACTTCGAGGAAGATGAAGAGGACACATACTACACTAAGGACCTCCCAGTCCACGC CTGCAGTTACTGTGGAATCCATGATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGC AATGGCCGAGGAAATACTTCTGGCAGCCACATTGTGAATCACCTCGTGAGGGCAAAATGCAAGGAAGTGA CGCTGCACAAGGACGGGCCTCTGGGCGAGACCGTGCTGGAGTGCTACAACTGTGGCTGCCGCAACGTCTT CCTGCTGGGCTTCATCCCTGCGAAGGCCGACTCTGTGGTGGTGCTGTTGTGCAGGCAGCCCTGTGCCAGC CAGAGCAGCCTGAAGGACATCAACTGGGACAGCTCACAGTGGCAGCCCCTAATCCAGGACCGGTGCTTTC TGTCATGGCTGGTCAAGATTCCCTCTGAGCAGGAGCAGCTGCGAGCACGGCAGATCACGGCACAGCAGAT CAACAAGCTGGAAGAGCTCTGGAAGGAAAATCCTTCAGCCACTCTGGAGGACCTGGAGAAGCCAGGCGTA GACGAGGAGCCACAGCACGTGCTCCTGCGTTACGAGGATGCTTACCAGTACCAGAACATCTTCGGGCCAC TGGTCAAGCTGGAGGCTGACTATGACAAGAAGTTGAAGGAGTCACAGACTCAAGATAACATCACGGTCAG GTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTTCTTCACTTTGCCCAAGACTGACTCTGACATGCGG CTCATGCAGGGTGATGAGATCTGTCTGCGGTACAAAGGGGATCTGGCGCCCCTGTGGAAGGGGATTGGCC ACGTCATCAAGGTTCCTGATAATTATGGTGATGAGATTGCTATTGAGCTCCGCAGCAGCGTGGGTGCCCC TGTGGAAGTGACCCACAACTTCCAAGTGGATTTTGTGTGGAAGTCAACCTCTTTTGATAGGATGCAGAGT GCACTGAAGACCTTCGCTGTGGACGAGACCTCTGTGTCAGGGTATATTTACCACAAGCTGCTGGGCCACG AGGTGGAGGATGTGGTCATCAAGTGCCAGCTGCCAAAGCGCTTCACAGCTCAGGGGCTCCCTGACCTCAA CCACTCTCAGGTGTATGCTGTGAAGACCGTGCTGCAGAGACCACTCAGCCTCATCCAGGGCCCTCCAGGC ACAGGCAAGACTGTGACATCAGCCACTATTGTCTACCACCTTGCTCGGCAGGGCAATGGGCCTGTACTGG TTTGTGCTCCAAGTAACATCGCTGTGGACCAGCTCACAGAGAAGATCCACCAGACAGGACTGAAGGTCGT ACGCCTCTGTGCCAAGAGCCGTGAGGCCATTGACTCCCCAGTGTCCTTCCTGGCTTTGCACAACCAGATC AGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGCAGCAGCTAAAGGATGAGACAGGCGAGCTGTCAT CTGCAGATGAGAAGCGGTACCGGGCGCTTAAGCGCACAGCTGAGAGAGAACTTCTCATGAATGCAGATGT CATATGCTGCACATGTGTGGGTGCTGGTGACCCGAGGCTGGCCAAGATGCAGTTCCGTTCCATCCTCATT GACGAGAGCACCCAGGCCACTGAGCCTGAGTGCATGGTGCCTGTAGTCCTTGGGGCCAAGCAGCTAATCC TCGTCGGTGACCACTGCCAGCTGGGCCCAGTGGTGATGTGCAAGAAGGCAGCCAAGGCCGGACTGTCACA ATCGCTCTTCGAGCGCTTGGTGGTGCTGGGCATCCGGCCCATCCGCCTGCAGGTGCAATACCGCATGCAC CCTGCACTCAGCGCCTTTCCGTCCAACATCTTCTACGAGGGCTCATTGCAGAATGGCGTCACTGCAGCGG ATCGTGTCAAAAAAGGCTTTGACTTCCAGTGGCCACAACCTGACAAGCCTATGTTCTTCTACGTGACGCA GGGCCAGGAGGAGATTGCCAGCTCTGGCACATCCTACCTCAACAGGACGGAGGCAGCCAATGTGGAGAAG ATAACTACGAAGCTGTTGAAGGCAGGTGCAAAGCCTGACCAGATCGGCATCATCACCCCCTACGAGGGCC AGCGCTCTTACTTGGTGCAGTACATGCAGTTCAGCGGCTCCCTGCACACAAAGCTCTACCAGGAAGTGGA AATTGCCAGTGTGGACGCCTTCCAGGGCCGGGAGAAGGACTTCATCATTCTGTCCTGCGTGCGCGCCAAT GAACATCAGGGCATTGGGTTCCTAAACGACCCCCGGCGTCTGAATGTGGCTCTCACCAGAGCAAGATATG GCGTGATCATTGTGGGTAACCCAAAGGCCCTGTCGAAGCAGCCCCTGTGGAATCACCTGCTGAGCTACTA CAAGGAACAGAAGGCGCTAGTGGAAGGGCCGCTCAACAACCTACGTGAGAGCCTCATGCAGTTCAGCAAG CCTCGCAAACTTGTCAACACTGTCAACCCGGGTGCCCGCTTCATGACTACTGCCATGTACGATGCCCGTG AGGCCATCATCCCCGGGTCTGTCTATGACCGCAGCAGCCAGGGCCGGCCCTCGAACATGTACTTCCAGAC CCATGACCAGATTAGTATGATCAGCGCAGGCCCCAGCCACGTGGCTGCCATGAACATCCCTATTCCCTTC AACTTGGTCATGCCTCCCATGCCGCCACCTGGCTACTTCGGACAGGCCAACGGGCCGGCAGCTGGTCGGG GCACCCCAAAAACCAAGACTGGCCGTGGGGGCCGCCAGAAGAACCGCTTTGGGCTTCCTGGGCCCAGCCA GACCACCCTTCCCAACAGCCAGGCCAGCCAGGACGTGGCCTCCCAGCCCTTTTCACAGGGTGCCCTCACA CAGGGTTACGTGTCCATGAGCCAGCCCTCTCAGATGAGCCAGCCTGGCCTCTCCCAGCCAGAACTGTCCC AGGACAGCTACCTCGGTGATGAGTTTAAATCACAGATTGACGTGGCACTCTCACAAGACTCCACATACCA GGGAGAGCGGGCATACCAGCACGGCGGGGTCACCGGGCTGTCCCAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211651 representing NM_030680
Red=Cloning site Green=Tags(s) MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGAGGPGGAGAGGAAGQLD AQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFC NGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCAS QSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGV DEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAFFTLPKTDSDMR LMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQS ALKTFAVDETSVSGYIYHKLLGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPG TGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQI RNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILI DESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMH PALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEK ITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRAN EHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLSYYKEQKALVEGPLNNLRESLMQFSK PRKLVNTVNPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQISMISAGPSHVAAMNIPIPF NLVMPPMPPPGYFGQANGPAAGRGTPKTKTGRGGRQKNRFGLPGPSQTTLPNSQASQDVASQPFSQGALT QGYVSMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030680 |
| ORF Size | 3339 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030680.3 |
| RefSeq Size | 4598 bp |
| RefSeq ORF | 3342 bp |
| Locus ID | 19704 |
| UniProt ID | Q9EPU0 |
| MW | 123.1 kDa |
| Gene Summary | RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223607 | Upf1 (untagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2, (10ug) |
CNY 14,250.00 |
|
| MG211651 | Upf1 (tGFP-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1) |
CNY 12,260.00 |
|
| MR211651L3 | Lenti ORF clone of Upf1 (Myc-DDK-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2 |
CNY 13,020.00 |
|
| MR211651L4 | Lenti ORF clone of Upf1 (mGFP-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2 |
CNY 13,020.00 |
