RNA-dependent RNA polymerase NS5 (NC_001474) Virus Tagged ORF Clone

CAT＃: VC102552

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for RNA-dependent RNA polymerase NS5 [Dengue virus 2], codon optimized for human cell expression, NP_739590

Datasheet

SDS

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CNY 13,210.00

货期*

3周

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DENV_gp1, Dengue, DENV
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102552 represents NCBI reference of NP_739590 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTACAGGAAATATAGGGGAGACTCTTGGTGAAAAGTGGAAGTCTAGACTGAACGCCCTTGGAAAAT CTGAGTTTCAGATCTACAAAAAGTCCGGAATCCAGGAGGTTGATCGAACACTCGCCAAGGAAGGAATCAA ACGGGGAGAGACCGACCATCATGCAGTATCACGCGGGTCTGCTAAGCTTCGCTGGTTCGTAGAACGAAAC ATGGTGACCCCCGAAGGCAAAGTCGTGGACCTTGGGTGCGGTCGGGGCGGCTGGAGCTATTACTGTGGAG GATTGAAAAACGTTCGGGAAGTGAAAGGCCTGACTAAGGGAGGACCAGGACACGAGGAACCTATTCCCAT GAGCACATACGGCTGGAACCTGGTACGACTTCAATCAGGTGTTGACGTGTTCTTCATCCCACCAGAGAAA TGTGATACTCTGCTCTGTGACATAGGAGAGTCTAGCCCCAATCCTACTGTTGAGGCCGGCAGAACCCTTC GCGTTTTGAACCTCGTGGAAAACTGGCTGAATAACAATACCCAATTTTGCATTAAAGTGCTTAATCCATA CATGCCCTCAGTAATAGAAAAGATGGAGGCCTTGCAACGAAAATACGGGGGCGCACTCGTAAGGAATCCT TTGTCTCGCAATTCAACCCACGAAATGTACTGGGTCTCCAACGCATCCGGAAACATTGTTAGTTCCGTGA ATATGATTTCCAGAATGCTGATTAACAGATTCACAATGCGGTACAAGAAAGCAACATACGAACCAGATGT AGACCTCGGCTCTGGAACTCGCAACATTGGCATTGAGTCTGAAATACCCAATCTGGACATTATCGGAAAA AGAATCGAGAAAATCAAACAAGAACATGAGACTTCCTGGCACTACGATCAGGACCATCCCTATAAAACCT GGGCTTACCACGGATCATATGAGACCAAGCAAACTGGATCCGCAAGTTCAATGGTTAACGGCGTAGTACG GCTTCTTACCAAACCATGGGATGTCGTTCCCATGGTGACCCAGATGGCAATGACTGATACAACACCATTC GGCCAGCAGCGAGTTTTTAAGGAGAAAGTCGACACACGCACTCAAGAGCCCAAAGAGGGTACAAAAAAGC TCATGAAAATAACAGCGGAGTGGTTGTGGAAAGAACTGGGCAAGAAAAAAACCCCTCGGATGTGTACTCG CGAAGAATTCACCCGGAAGGTGCGGAGTAATGCAGCCCTCGGGGCAATCTTTACTGACGAAAATAAATGG AAGAGCGCACGAGAAGCCGTCGAAGATTCACGATTCTGGGAACTGGTTGATAAAGAACGAAACTTGCATC TGGAAGGTAAATGCGAAACTTGCGTGTATAACATGATGGGGAAGCGCGAAAAAAAGCTGGGAGAATTCGG TAAAGCAAAGGGCAGCAGAGCTATTTGGTACATGTGGTTGGGCGCAAGGTTTCTGGAATTTGAGGCCCTG GGATTTTTGAATGAGGATCATTGGTTTAGTAGGGAGAATAGTTTGTCTGGCGTGGAGGGTGAAGGGCTTC ACAAGCTCGGCTACATCCTTAGAGATGTGAGCAAAAAAGAAGGAGGTGCTATGTACGCTGACGATACAGC TGGCTGGGACACACGCATTACACTCGAAGACCTGAAGAATGAAGAAATGGTAACAAATCACATGGAAGGA GAGCATAAAAAGCTCGCAGAGGCAATTTTTAAACTGACGTACCAAAATAAGGTGGTTCGAGTACAGCGCC CAACCCCACGAGGCACAGTAATGGATATAATTAGTCGCCGGGATCAGCGAGGATCAGGACAGGTTGGAAC ATACGGATTGAATACTTTCACCAACATGGAGGCACAACTGATCAGACAGATGGAAGGTGAAGGCGTTTTT AAGAGCATTCAGCACTTGACAATTACCGAGGAGATTGCGGTACAAAACTGGTTGGCCAGGGTCGGCAGGG AACGGCTTAGCAGAATGGCCATTTCAGGGGATGACTGTGTGGTTAAACCTCTCGACGATAGATTTGCTTC CGCTTTGACCGCTTTGAACGACATGGGTAAGATTCGCAAAGACATCCAACAGTGGGAACCAAGTCGGGGT TGGAATGATTGGACACAGGTGCCTTTCTGCTCTCATCATTTTCATGAGTTGATAATGAAAGATGGTCGCG TACTGGTTGTCCCATGTAGAAACCAGGACGAACTTATTGGAAGGGCCAGGATAAGCCAAGGAGCTGGATG GTCCCTGAGAGAGACTGCATGCCTCGGCAAATCATACGCCCAAATGTGGTCCCTCATGTATTTCCATAGG AGAGATCTCAGACTGGCAGCCAACGCCATTTGCTCTGCCGTTCCCTCACATTGGGTTCCGACAAGTAGGA CGACATGGTCTATTCACGCCAAACACGAGTGGATGACTACAGAGGATATGCTGACTGTGTGGAATCGCGT TTGGATTCAGGAAAACCCATGGATGGAGGATAAGACTCCCGTGGAGAGTTGGGAAGAAATCCCCTACTTG GGGAAAAGAGAAGATCAGTGGTGCGGTTCACTTATCGGCTTGACCTCTAGGGCCACTTGGGCCAAAAATA TTCAAGCTGCCATCAATCAGGTCAGGAGCCTGATTGGAAACGAGGAATACACAGATTACATGCCATCCAT GAAAAGATTTAGAAGGGAGGAGGAGGAGGCGGGAGTGTTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102552 representing NP_739590 Red=Cloning sites Green=Tags MGTGNIGETLGEKWKSRLNALGKSEFQIYKKSGIQEVDRTLAKEGIKRGETDHHAVSRGSAKLRWFVERN MVTPEGKVVDLGCGRGGWSYYCGGLKNVREVKGLTKGGPGHEEPIPMSTYGWNLVRLQSGVDVFFIPPEK CDTLLCDIGESSPNPTVEAGRTLRVLNLVENWLNNNTQFCIKVLNPYMPSVIEKMEALQRKYGGALVRNP LSRNSTHEMYWVSNASGNIVSSVNMISRMLINRFTMRYKKATYEPDVDLGSGTRNIGIESEIPNLDIIGK RIEKIKQEHETSWHYDQDHPYKTWAYHGSYETKQTGSASSMVNGVVRLLTKPWDVVPMVTQMAMTDTTPF GQQRVFKEKVDTRTQEPKEGTKKLMKITAEWLWKELGKKKTPRMCTREEFTRKVRSNAALGAIFTDENKW KSAREAVEDSRFWELVDKERNLHLEGKCETCVYNMMGKREKKLGEFGKAKGSRAIWYMWLGARFLEFEAL GFLNEDHWFSRENSLSGVEGEGLHKLGYILRDVSKKEGGAMYADDTAGWDTRITLEDLKNEEMVTNHMEG EHKKLAEAIFKLTYQNKVVRVQRPTPRGTVMDIISRRDQRGSGQVGTYGLNTFTNMEAQLIRQMEGEGVF KSIQHLTITEEIAVQNWLARVGRERLSRMAISGDDCVVKPLDDRFASALTALNDMGKIRKDIQQWEPSRG WNDWTQVPFCSHHFHELIMKDGRVLVVPCRNQDELIGRARISQGAGWSLRETACLGKSYAQMWSLMYFHR RDLRLAANAICSAVPSHWVPTSRTTWSIHAKHEWMTTEDMLTVWNRVWIQENPWMEDKTPVESWEEIPYL GKREDQWCGSLIGLTSRATWAKNIQAAINQVRSLIGNEEYTDYMPSMKRFRREEEEAGVLW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_001474
ORF Size	2703 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NC_001474.2, NP_739590
RefSeq ORF	2703 bp
MW	103.4 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC102541	Myc-DDK-tagged ORF clone of viral ORF for anchored capsid protein C [Dengue virus 2], codon optimized for human cell expression, NP_739581	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102542	Myc-DDK-tagged ORF clone of viral ORF for capsid protein C [Dengue virus 2], codon optimized for human cell expression, NP_739591	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102543	Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein precursor M [Dengue virus 2], codon optimized for human cell expression, NP_739582	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,640.00 CNY 3,800.00
VC102544	Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein M [Dengue virus 2], codon optimized for human cell expression, NP_739592	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102545	Myc-DDK-tagged ORF clone of viral ORF for envelope protein E [Dengue virus 2], codon optimized for human cell expression, NP_739583	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 5,800.00
VC102546	Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS1 [Dengue virus 2], codon optimized for human cell expression, NP_739584	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,180.00
VC102547	Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2A [Dengue virus 2], codon optimized for human cell expression, NP_739585	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102548	Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2B [Dengue virus 2], codon optimized for human cell expression, NP_739586	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102549	Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS3 [Dengue virus 2], codon optimized for human cell expression, NP_739587	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,320.00
VC102550	Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4A [Dengue virus 2], codon optimized for human cell expression, NP_739588	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102551	Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4B [Dengue virus 2], codon optimized for human cell expression, NP_739589	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102553	Myc-DDK-tagged ORF clone of viral ORF for propein pr [Dengue virus 2], codon optimized for human cell expression, YP_009164954	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102554	Myc-DDK-tagged ORF clone of viral ORF for protein 2K [Dengue virus 2], codon optimized for human cell expression, NP_739593	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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