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RNA-dependent RNA polymerase NS5 (NC_001474) Virus Tagged ORF Clone
CAT＃: VC102552
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for RNA-dependent RNA polymerase NS5 [Dengue virus 2], codon optimized for human cell expression, NP_739590
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CNY 13,210.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DENV_gp1, Dengue, DENV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102552 represents NCBI reference of NP_739590 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTACAGGAAATATAGGGGAGACTCTTGGTGAAAAGTGGAAGTCTAGACTGAACGCCCTTGGAAAAT CTGAGTTTCAGATCTACAAAAAGTCCGGAATCCAGGAGGTTGATCGAACACTCGCCAAGGAAGGAATCAA ACGGGGAGAGACCGACCATCATGCAGTATCACGCGGGTCTGCTAAGCTTCGCTGGTTCGTAGAACGAAAC ATGGTGACCCCCGAAGGCAAAGTCGTGGACCTTGGGTGCGGTCGGGGCGGCTGGAGCTATTACTGTGGAG GATTGAAAAACGTTCGGGAAGTGAAAGGCCTGACTAAGGGAGGACCAGGACACGAGGAACCTATTCCCAT GAGCACATACGGCTGGAACCTGGTACGACTTCAATCAGGTGTTGACGTGTTCTTCATCCCACCAGAGAAA TGTGATACTCTGCTCTGTGACATAGGAGAGTCTAGCCCCAATCCTACTGTTGAGGCCGGCAGAACCCTTC GCGTTTTGAACCTCGTGGAAAACTGGCTGAATAACAATACCCAATTTTGCATTAAAGTGCTTAATCCATA CATGCCCTCAGTAATAGAAAAGATGGAGGCCTTGCAACGAAAATACGGGGGCGCACTCGTAAGGAATCCT TTGTCTCGCAATTCAACCCACGAAATGTACTGGGTCTCCAACGCATCCGGAAACATTGTTAGTTCCGTGA ATATGATTTCCAGAATGCTGATTAACAGATTCACAATGCGGTACAAGAAAGCAACATACGAACCAGATGT AGACCTCGGCTCTGGAACTCGCAACATTGGCATTGAGTCTGAAATACCCAATCTGGACATTATCGGAAAA AGAATCGAGAAAATCAAACAAGAACATGAGACTTCCTGGCACTACGATCAGGACCATCCCTATAAAACCT GGGCTTACCACGGATCATATGAGACCAAGCAAACTGGATCCGCAAGTTCAATGGTTAACGGCGTAGTACG GCTTCTTACCAAACCATGGGATGTCGTTCCCATGGTGACCCAGATGGCAATGACTGATACAACACCATTC GGCCAGCAGCGAGTTTTTAAGGAGAAAGTCGACACACGCACTCAAGAGCCCAAAGAGGGTACAAAAAAGC TCATGAAAATAACAGCGGAGTGGTTGTGGAAAGAACTGGGCAAGAAAAAAACCCCTCGGATGTGTACTCG CGAAGAATTCACCCGGAAGGTGCGGAGTAATGCAGCCCTCGGGGCAATCTTTACTGACGAAAATAAATGG AAGAGCGCACGAGAAGCCGTCGAAGATTCACGATTCTGGGAACTGGTTGATAAAGAACGAAACTTGCATC TGGAAGGTAAATGCGAAACTTGCGTGTATAACATGATGGGGAAGCGCGAAAAAAAGCTGGGAGAATTCGG TAAAGCAAAGGGCAGCAGAGCTATTTGGTACATGTGGTTGGGCGCAAGGTTTCTGGAATTTGAGGCCCTG GGATTTTTGAATGAGGATCATTGGTTTAGTAGGGAGAATAGTTTGTCTGGCGTGGAGGGTGAAGGGCTTC ACAAGCTCGGCTACATCCTTAGAGATGTGAGCAAAAAAGAAGGAGGTGCTATGTACGCTGACGATACAGC TGGCTGGGACACACGCATTACACTCGAAGACCTGAAGAATGAAGAAATGGTAACAAATCACATGGAAGGA GAGCATAAAAAGCTCGCAGAGGCAATTTTTAAACTGACGTACCAAAATAAGGTGGTTCGAGTACAGCGCC CAACCCCACGAGGCACAGTAATGGATATAATTAGTCGCCGGGATCAGCGAGGATCAGGACAGGTTGGAAC ATACGGATTGAATACTTTCACCAACATGGAGGCACAACTGATCAGACAGATGGAAGGTGAAGGCGTTTTT AAGAGCATTCAGCACTTGACAATTACCGAGGAGATTGCGGTACAAAACTGGTTGGCCAGGGTCGGCAGGG AACGGCTTAGCAGAATGGCCATTTCAGGGGATGACTGTGTGGTTAAACCTCTCGACGATAGATTTGCTTC CGCTTTGACCGCTTTGAACGACATGGGTAAGATTCGCAAAGACATCCAACAGTGGGAACCAAGTCGGGGT TGGAATGATTGGACACAGGTGCCTTTCTGCTCTCATCATTTTCATGAGTTGATAATGAAAGATGGTCGCG TACTGGTTGTCCCATGTAGAAACCAGGACGAACTTATTGGAAGGGCCAGGATAAGCCAAGGAGCTGGATG GTCCCTGAGAGAGACTGCATGCCTCGGCAAATCATACGCCCAAATGTGGTCCCTCATGTATTTCCATAGG AGAGATCTCAGACTGGCAGCCAACGCCATTTGCTCTGCCGTTCCCTCACATTGGGTTCCGACAAGTAGGA CGACATGGTCTATTCACGCCAAACACGAGTGGATGACTACAGAGGATATGCTGACTGTGTGGAATCGCGT TTGGATTCAGGAAAACCCATGGATGGAGGATAAGACTCCCGTGGAGAGTTGGGAAGAAATCCCCTACTTG GGGAAAAGAGAAGATCAGTGGTGCGGTTCACTTATCGGCTTGACCTCTAGGGCCACTTGGGCCAAAAATA TTCAAGCTGCCATCAATCAGGTCAGGAGCCTGATTGGAAACGAGGAATACACAGATTACATGCCATCCAT GAAAAGATTTAGAAGGGAGGAGGAGGAGGCGGGAGTGTTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102552 representing NP_739590
Red=Cloning sites Green=Tags MGTGNIGETLGEKWKSRLNALGKSEFQIYKKSGIQEVDRTLAKEGIKRGETDHHAVSRGSAKLRWFVERN MVTPEGKVVDLGCGRGGWSYYCGGLKNVREVKGLTKGGPGHEEPIPMSTYGWNLVRLQSGVDVFFIPPEK CDTLLCDIGESSPNPTVEAGRTLRVLNLVENWLNNNTQFCIKVLNPYMPSVIEKMEALQRKYGGALVRNP LSRNSTHEMYWVSNASGNIVSSVNMISRMLINRFTMRYKKATYEPDVDLGSGTRNIGIESEIPNLDIIGK RIEKIKQEHETSWHYDQDHPYKTWAYHGSYETKQTGSASSMVNGVVRLLTKPWDVVPMVTQMAMTDTTPF GQQRVFKEKVDTRTQEPKEGTKKLMKITAEWLWKELGKKKTPRMCTREEFTRKVRSNAALGAIFTDENKW KSAREAVEDSRFWELVDKERNLHLEGKCETCVYNMMGKREKKLGEFGKAKGSRAIWYMWLGARFLEFEAL GFLNEDHWFSRENSLSGVEGEGLHKLGYILRDVSKKEGGAMYADDTAGWDTRITLEDLKNEEMVTNHMEG EHKKLAEAIFKLTYQNKVVRVQRPTPRGTVMDIISRRDQRGSGQVGTYGLNTFTNMEAQLIRQMEGEGVF KSIQHLTITEEIAVQNWLARVGRERLSRMAISGDDCVVKPLDDRFASALTALNDMGKIRKDIQQWEPSRG WNDWTQVPFCSHHFHELIMKDGRVLVVPCRNQDELIGRARISQGAGWSLRETACLGKSYAQMWSLMYFHR RDLRLAANAICSAVPSHWVPTSRTTWSIHAKHEWMTTEDMLTVWNRVWIQENPWMEDKTPVESWEEIPYL GKREDQWCGSLIGLTSRATWAKNIQAAINQVRSLIGNEEYTDYMPSMKRFRREEEEAGVLW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001474 |
| ORF Size | 2703 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001474.2, NP_739590 |
| RefSeq ORF | 2703 bp |
| MW | 103.4 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102541 | Myc-DDK-tagged ORF clone of viral ORF for anchored capsid protein C [Dengue virus 2], codon optimized for human cell expression, NP_739581 |
CNY 3,800.00 |
|
| VC102542 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein C [Dengue virus 2], codon optimized for human cell expression, NP_739591 |
CNY 3,800.00 |
|
| VC102543 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein precursor M [Dengue virus 2], codon optimized for human cell expression, NP_739582 |
CNY 2,640.00 |
|
| VC102544 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein M [Dengue virus 2], codon optimized for human cell expression, NP_739592 |
CNY 3,800.00 |
|
| VC102545 | Myc-DDK-tagged ORF clone of viral ORF for envelope protein E [Dengue virus 2], codon optimized for human cell expression, NP_739583 |
CNY 3,656.00 |
|
| VC102546 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS1 [Dengue virus 2], codon optimized for human cell expression, NP_739584 |
CNY 4,180.00 |
|
| VC102547 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2A [Dengue virus 2], codon optimized for human cell expression, NP_739585 |
CNY 3,800.00 |
|
| VC102548 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2B [Dengue virus 2], codon optimized for human cell expression, NP_739586 |
CNY 3,800.00 |
|
| VC102549 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS3 [Dengue virus 2], codon optimized for human cell expression, NP_739587 |
CNY 7,320.00 |
|
| VC102550 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4A [Dengue virus 2], codon optimized for human cell expression, NP_739588 |
CNY 3,800.00 |
|
| VC102551 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4B [Dengue virus 2], codon optimized for human cell expression, NP_739589 |
CNY 3,800.00 |
|
| VC102553 | Myc-DDK-tagged ORF clone of viral ORF for propein pr [Dengue virus 2], codon optimized for human cell expression, YP_009164954 |
CNY 3,800.00 |
|
| VC102554 | Myc-DDK-tagged ORF clone of viral ORF for protein 2K [Dengue virus 2], codon optimized for human cell expression, NP_739593 |
CNY 3,800.00 |
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