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nonstructural protein NS3 (NC_012532) Virus Tagged ORF Clone
CAT＃: VC102534
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS3 [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227202.1
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CNY 5,064.00
CNY 7,220.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ZIKV_gp1, ZIKV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102534 represents NCBI reference of YP_009227202 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGCGCACTGTGGGACGTGCCCGCCCCAAAGGAAGTCAAGAAGGGGGAAACTACCGATGGCGTAT ATAGAGTTATGACAAGAAGACTGCTGGGCTCCACACAGGTAGGAGTGGGCGTGATGCAGGAAGGAGTTTT TCATACTATGTGGCACGTGACTAAGGGCGCAGCACTGCGAAGTGGAGAGGGAAGGCTTGATCCTTATTGG GGCGATGTGAAACAGGACCTGGTTTCATACTGCGGGCCGTGGAAGTTGGATGCTGCGTGGGACGGCCTGT CTGAAGTTCAGCTCCTTGCTGTGCCTCCCGGAGAACGAGCTAGGAATATTCAAACTCTGCCAGGTATCTT TAAGACAAAGGACGGAGACATTGGAGCCGTTGCCCTTGACTACCCAGCCGGCACCAGCGGTAGTCCAATC CTCGACAAGTGCGGCCGGGTCATCGGACTTTATGGCAATGGAGTGGTGATTAAAAATGGATCATACGTGA GCGCTATAACACAAGGAAAGAGAGAAGAAGAAACCCCAGTTGAATGCTTTGAACCGAGCATGCTCAAGAA GAAACAGCTTACCGTGCTTGATTTGCATCCAGGCGCTGGGAAGACTCGCCGCGTTCTCCCTGAAATCGTT AGAGAAGCCATCAAGAAGCGCTTGAGAACTGTCATCCTTGCACCCACAAGAGTCGTCGCCGCCGAAATGG AAGAGGCTCTCCGAGGTCTTCCAGTCCGGTACATGACAACCGCCGTTAACGTGACACACTCTGGAACCGA AATTGTTGACCTTATGTGCCACGCCACGTTCACTTCCAGACTTCTCCAACCTATCAGGGTGCCAAACTAC AATCTTAACATCATGGACGAAGCACATTTTACTGACCCCTCAAGTATCGCGGCCCGGGGTTATATTAGCA CAAGAGTCGAGATGGGAGAAGCCGCCGCAATCTTTATGACCGCCACTCCACCAGGAACCCGGGATGCCTT CCCCGATAGCAACTCACCCATCATGGACACAGAGGTCGAAGTGCCTGAGCGCGCCTGGTCTTCAGGATTC GACTGGGTCACTGACCACTCCGGGAAAACCGTATGGTTCGTACCATCCGTTAGAAATGGCAACGAGATTG CAGCTTGTCTCACTAAGGCTGGCAAGAGGGTGATCCAGCTTTCCCGAAAGACCTTCGAGACTGAATTTCA GAAGACCAAAAACCAGGAATGGGACTTCGTGATTACTACCGATATCAGCGAGATGGGGGCCAACTTTAAA GCTGACAGAGTGATCGACAGCAGAAGGTGCCTGAAGCCCGTAATATTGGACGGCGAACGAGTCATTTTGG CAGGCCCAATGCCCGTGACCCATGCTTCAGCTGCACAGAGGCGGGGCAGGATCGGCCGGAACCCCAATAA GCCCGGTGACGAGTACATGTATGGAGGCGGATGCGCTGAAACTGACGAAGGACATGCACACTGGCTGGAA GCCAGAATGCTCCTGGATAATATATATCTCCAAGATGGTCTCATTGCAAGTCTTTACCGCCCTGAGGCTG ATAAGGTTGCTGCCATCGAAGGCGAGTTTAAGCTGAGGACAGAGCAAAGGAAAACTTTCGTGGAACTGAT GAAGAGAGGTGACCTGCCCGTGTGGCTGGCATACCAGGTGGCTAGTGCCGGAATTACCTACACAGACCGG AGATGGTGCTTTGATGGGACCACTAATAACACAATCATGGAAGATAGCGTGCCTGCCGAGGTCTGGACGA AGTACGGCGAGAAAAGAGTACTGAAACCCCGCTGGATGGATGCAAGAGTCTGTAGTGACCACGCCGCCCT GAAGAGCTTTAAAGAATTTGCGGCTGGGAAACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102534 representing YP_009227202
Red=Cloning sites Green=Tags MSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMWHVTKGAALRSGEGRLDPYW GDVKQDLVSYCGPWKLDAAWDGLSEVQLLAVPPGERARNIQTLPGIFKTKDGDIGAVALDYPAGTSGSPI LDKCGRVIGLYGNGVVIKNGSYVSAITQGKREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIV REAIKKRLRTVILAPTRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNY NLNIMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEVPERAWSSGF DWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKTKNQEWDFVITTDISEMGANFK ADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQRRGRIGRNPNKPGDEYMYGGGCAETDEGHAHWLE ARMLLDNIYLQDGLIASLYRPEADKVAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDR RWCFDGTTNNTIMEDSVPAEVWTKYGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_012532 |
| ORF Size | 1854 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_012532.1, YP_009227202 |
| RefSeq ORF | 1854 bp |
| MW | 68.5 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102527 | Myc-DDK-tagged ORF clone of viral ORF for anchored capsid protein C [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227206.1 |
CNY 3,800.00 |
|
| VC102528 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein C [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227196.1 |
CNY 1,320.00 |
|
| VC102529 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein precursor M [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227197.1 |
CNY 2,640.00 |
|
| VC102530 | Myc-DDK-tagged ORF clone of viral ORF for envelope protein E [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227198.1 |
CNY 5,968.00 |
|
| VC102531 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS1 [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227199.1 |
CNY 5,488.00 |
|
| VC102532 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2A [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227200.1 |
CNY 2,640.00 |
|
| VC102533 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2B [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227201.1 |
CNY 1,320.00 |
|
| VC102535 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4A [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227203.1 |
CNY 1,920.00 |
|
| VC102536 | Myc-DDK-tagged ORF clone of viral ORF for Protein 2K [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227209.1 |
CNY 3,800.00 |
|
| VC102537 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4B [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227204.1 |
CNY 3,600.00 |
|
| VC102538 | Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS5 [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227205.1 |
CNY 7,280.00 |
|
| VC102539 | Myc-DDK-tagged ORF clone of viral ORF for Membrane glycoprotein M [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227208.1 |
CNY 3,800.00 |
|
| VC102540 | Myc-DDK-tagged ORF clone of viral ORF for Protein pr [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227207.1 |
CNY 3,800.00 |
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