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VP4 (NC_009996) Virus Tagged ORF Clone
CAT＃: VC102130
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for VP4 [Human rhinovirus C], codon optimized for human cell expression, YP_001552432. Note: ORF is codon optimized
View other clones from "Virus" (9)
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>VC102130 representing YP_001552432, codon optimized.
Due to the complexity of YP_001552432, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGCCCAGGTGAGTAAGCAGAATGTGGGAAGTCATGAAAACTCTGTCAGCGCAACAGGCGGCAGT GTGATTAAATACTTTAACATTAATTACTACAAAGACAGCGCCAGCTCCGGACTCACTAAACAGGACTTC AGTCAGGACCCCAGCAAATTCACCCAGCCCTTGGCCGAAGCGCTGACAAACCCGGCACTGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >VC102130 representing YP_001552432
Blue=ORF Red=Cloning site Green=Tag(s) MGAQVSKQNVGSHENSVSATGGSVIKYFNINYYKDSASSGLTKQDFSQDPSKFTQPLAEALTNPALM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NC_009996 |
| ORF Size | 201 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | YP_001552432 |
| RefSeq ORF | 201 bp |
| MW | 7.2 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102131 | Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human rhinovirus C], codon optimized for human cell expression, YP_001552433 |
CNY 3,800.00 |
|
| VC102132 | Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human rhinovirus C], codon optimized for human cell expression, YP_001552434 |
CNY 3,800.00 |
|
| VC102133 | Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human rhinovirus C], codon optimized for human cell expression, YP_001552435 |
CNY 3,800.00 |
|
| VC102134 | Myc-DDK-tagged ORF clone of viral ORF for 2A [Human rhinovirus C], codon optimized for human cell expression, YP_001552436 |
CNY 3,800.00 |
|
| VC102135 | Myc-DDK-tagged ORF clone of viral ORF for 2B [Human rhinovirus C], codon optimized for human cell expression, YP_001552437 |
CNY 3,800.00 |
|
| VC102136 | Myc-DDK-tagged ORF clone of viral ORF for 2C [Human rhinovirus C], codon optimized for human cell expression, YP_001552438 |
CNY 2,400.00 |
|
| VC102137 | Myc-DDK-tagged ORF clone of viral ORF for 3A [Human rhinovirus C], codon optimized for human cell expression, YP_001552439 |
CNY 1,320.00 |
|
| VC102138 | Myc-DDK-tagged ORF clone of viral ORF for 3B [Human rhinovirus C], codon optimized for human cell expression, YP_001552440 |
CNY 3,800.00 |
|
| VC102139 | Myc-DDK-tagged ORF clone of viral ORF for 3C [Human rhinovirus C], codon optimized for human cell expression, YP_001552441 |
CNY 3,800.00 |
