VP2 (NC_015150) Virus Tagged ORF Clone
CAT#: VC102090
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243703
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CNY 4,180.00
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC102090 represents NCBI reference of YP_004243703 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGGCGTCTTGACCATCCTGCTCGACATCTTCGAACTGGCAGAGCAACTCAGCCTGTCTACGGGGT TCACCGTAGACGCAATTCTGAGCGGAGAAGCCATTGCAGCGGCCACTACTGAGGCAGCCTGGCTCATCGA GATCGAGGCTGTAGATGTGGCCGGGTTGGGGGCTCTCGAAGCATTGACCCTTACAGGACTCAGCGCAGAG GAGTTCTCACTGCTGTCCGCCCTCCCCACAGCCCTGAATAATGCTATCGGGATAGGGATCTTCTTTCAGA CTGTCACAGGCGCCAGCGCAGTGGTGGCCGCTGGGGTCACGACATTCGGGTATTCAAAAGAGGTGCCCCT GGTGAGCATGGCGCTGACGCCCTGGTTCCCACAGGTAGACTACTTGTTCCCAGGACTGTCCTCTTTCTCC TACTATCTGAACGCTGCCCTGGACTGGGGTGAATCACTGTTCCATGCCGTCGGTAGGGAAATTTGGCGGA ACATCATGAGACAGGCCACCCAGCAGATTGGGTATACATCCAGAGCTCTGGCAGTACGCGGAACCAACGA GTTTCAGCATATGCTGGCACAGATTGCTGAAAACGCACGATGGGCTCTCACTAATGGCCCAATCCACATT TACTCCAGCGTCGAAGAATATTATAGGGGGTTGCCCAGTGTTAATCCTATACAGTTGAGGCAGCAATACA GGTCTCGCGGCGAGCTGCCTCCTACCAGGGAGCAGTTTGAGTACCAGGAGCAAGTGCGGCTGAGACGGGA GATCGGCGGCAGCGAACCTAGATCAGGCCACTATGTCCAGCATTACGCTGCCCCAGGGGGGGCGAACCAA AGAGTGAGCCAGGACTGGATGCTCCCACTGATCCTTGGCCTTTACGGGGATATCACCCCAACATGGGAAA AGGAACTCTCCAAGCTTGAGAAGGAAGAGTACGGACCACCTAAGAAGAAAGCTCGGGTGAGAAGTATGTC CTGTAAAAAGAATTTGTCTAACACACGCAGTCGCTCTCAGACACCATGTCAGAGACGGGGCAGGTCCTCA AGGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102090 representing YP_004243703
Red=Cloning sites Green=Tags MGGVLTILLDIFELAEQLSLSTGFTVDAILSGEAIAAATTEAAWLIEIEAVDVAGLGALEALTLTGLSAE EFSLLSALPTALNNAIGIGIFFQTVTGASAVVAAGVTTFGYSKEVPLVSMALTPWFPQVDYLFPGLSSFS YYLNAALDWGESLFHAVGREIWRNIMRQATQQIGYTSRALAVRGTNEFQHMLAQIAENARWALTNGPIHI YSSVEEYYRGLPSVNPIQLRQQYRSRGELPPTREQFEYQEQVRLRREIGGSEPRSGHYVQHYAAPGGANQ RVSQDWMLPLILGLYGDITPTWEKELSKLEKEEYGPPKKKARVRSMSCKKNLSNTRSRSQTPCQRRGRSS RS TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_015150 |
ORF Size | 1056 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_015150.1, YP_004243703 |
RefSeq ORF | 1056 bp |
Locus ID | 10352036 |
MW | 38.9 kDa |
Documents
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Other Versions
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VC102089 | Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243705 |
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VC102091 | Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human polyomavirus 9], codon optimized for human cell expression, YP_004243704 |
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VC102092 | Myc-DDK-tagged ORF clone of viral ORF for large T antigen [Human polyomavirus 9], codon optimized for human cell expression, YP_004243706 |
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VC102093 | Myc-DDK-tagged ORF clone of viral ORF for small T antigen [Human polyomavirus 9], codon optimized for human cell expression, YP_004243707 |
CNY 3,800.00 |