VP1 (NC_015150) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102089 represents NCBI reference of YP_004243705 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCCAAAGAAAGAGACAAGAATGCGGCGCATGTCCTGTGAAAAAGACGTGTCCAACTCCTGCCC CCGTGCCTAAACTGCTCGTGAAGGGGGGCGTCGAAGTTCTGGAGGTTAGAACGGGACCAGATGCGATCAC CCAGATCGAGGCATATCTGAACCCACGGATGGGCAACAACAACCCTACCGACGAGCTGTATGGGTACTCC GCGGACATCAACGTCGCTTCCAGCAAAGCAAGCGACAATCCTAATGCAACAACCCTTCCTACATATTCTG TCGCTGTAATAAAGCTGCCAATGCTCAACGAAGATATGACATGTGACACCCTGTTGATGTGGGAGGCAGT CTCCGTCAAGACCGAGGTTATGGGAATCAGCAGTCTGGTCAACTTGCATCAGGGTGGCAAATATATTTAT GGCTCTTCTTCTGGTACCATACCCGTTCAGGGCACTACTCTTCACATGTTTAGTGTCGGCGGCGAACCAC TCGAGCTTCAGGGGCTCGTGGCATCTTCCACAACTACATACCCGACCGACATGGTGACTATTAAGAATAT GAAGCCCGTGAACCAGGCTCTGGACCCTAATGCTAAGGCGCTTCTGGACAAGGATGGGAAGTATCCGGTG GAAGTCTGGAGCCCCGATCCATCCAAAAACGAGAATACCCGCTACTATGGAAGCTTTACAGGGGGCGCCA CCACCCCACCAGTCATGCAGTTCACGAATTCCGTGACAACTGTGCTGCTGGATGAGAACGGCGTGGGTCC CCTGTGTAAAGGAGACAAGCTCTTCCTTAGTGCGGTGGATATCGTCGGGATCCATACCAATTATTCCGAA TCACAGAACTGGAGGGGGCTGCCCCGGTATTTCAACGTTACGCTCAGAAAGAGAGTGGTCAAAAATCCTT ATCCCGTTTCAAGTTTGCTCAATTCCCTGTTCTCAGGGCTGATGCCACAGATCCAGGGACAACCAATGGA GGGGACATCTGGACAAGTCGAGGAAGTGAGAATCTACCAGGGAACCGAGGGCCTGCCCGGCGACCCCGAC TTGGACCGGTATGTGGACAAGTTCTGTCAGAACCAAACCGTGCCCCCCCGAAGTAACGATCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102089 representing YP_004243705 Red=Cloning sites Green=Tags MAPQRKRQECGACPVKKTCPTPAPVPKLLVKGGVEVLEVRTGPDAITQIEAYLNPRMGNNNPTDELYGYS ADINVASSKASDNPNATTLPTYSVAVIKLPMLNEDMTCDTLLMWEAVSVKTEVMGISSLVNLHQGGKYIY GSSSGTIPVQGTTLHMFSVGGEPLELQGLVASSTTTYPTDMVTIKNMKPVNQALDPNAKALLDKDGKYPV EVWSPDPSKNENTRYYGSFTGGATTPPVMQFTNSVTTVLLDENGVGPLCKGDKLFLSAVDIVGIHTNYSE SQNWRGLPRYFNVTLRKRVVKNPYPVSSLLNSLFSGLMPQIQGQPMEGTSGQVEEVRIYQGTEGLPGDPD LDRYVDKFCQNQTVPPRSNDQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_015150
ORF Size	1113 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_015150.1, YP_004243705
RefSeq ORF	1113 bp
Locus ID	10352034
MW	40.3 kDa

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