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N (NC_003461) Virus Tagged ORF Clone
CAT＃: VC102042
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604433
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CNY 6,370.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102042 represents NCBI reference of NP_604433 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGTCTCCTGTCCACCTTTGATACCTTCTCTAGTCGCCGAAGCGAGTCCATCAACAAGAGCGGTG GAGGTGCCATCATCCCTGGCCAGCGCTCCACTGTGAGTGTGTTCACCCTCGGACCTTCAGTGACTGACGA TGCGGATAAGCTGTTGATCGCAACAACATTCCTGGCACACTCTCTTGATACCGATAAGCAGCACAGCCAA CGGGGCGGTTTTCTGGTCTCTCTGCTGGCCATGGCGTATAGCTCACCTGAGCTGTACCTCACTACTAATG GCGTCAATGCGGACGTCAAGTACGTAATCTATAATATTGAGAGAGACCCTAAACGCACTAAAACAGACGG ATTTATAGTCAAGACCAGAGATATGGAGTATGAGAGGACTACAGAGTGGCTCTTTGGCCCAATGATCAAC AAGAACCCTCTCTTCCAGGGCCAGCGGGAGAACGCCGATCTTGAAGCACTTCTGCAGACTTACGGATATC CTGCATGCCTCGGGGCCATTATTGTTCAAGTGTGGATCGTGCTGGTTAAGGCTATCACATCAAGCGCGGG GCTGAGGAAGGGTTTTTTTAATAGGCTGGAAGCGTTCAGACAGGACGGAACTGTAAAATCAGCTCTGGTC TTCACCGGAGATACCGTGGAAGGAATTGGGGCTGTAATGCGCAGCCAGCAGTCCTTGGTGAGTCTCATGG TGGAAACCCTGGTCACCATGAATACAAGCCGATCTGACTTGACCACCCTTGAGAAGAATATTCAGATCGT GGGCAATTACATCCGGGACGCCGGGCTGGCAAGTTTTATGAATACAATCAAGTACGGGGTCGAGACCAAA ATGGCTGCTCTGACCCTGAGTAACCTCAGGCCTGATATCAACAAACTGAGGTCCCTCGTAGACATTTATC TGTCAAAGGGCGCTCGGGCTCCATTCATCTGCATCCTCAGAGACCCAGTTCATGGAGACTTCGCCCCCGG TAACTATCCCGCGCTTTGGTCCTATGCTATGGGAGTTGCTGTTGTCCAGAACAAAGCTATGCAGCAATAT GTAACTGGCCGCACGTACCTTGATATGGAGATGTTCCTGCTGGGGCAGGCCGTGGCGAAAGATGCCGACT CCAAGATTAGTTCAGCTCTTGAAGAAGAGCTGGGCGTGACTGATACTGCCAAGGAGCGGCTGCGCCATCA CCTTACAAACCTCTCCGGTGGCGATGGCGCCTACCACAAGCCCACAGGCGGAGGGGCAATCGAAGTGGCC ATCGACCATACAGATATTACCTTCGGAGTTGAGGATACTGCAGATAGAGACAATAAGAACTGGACTAATG ATTCTAATGAAAGATGGATGAACCATAGCATAAGCAACCATACTATCACTATCCGGGGAGCTGAGGAGCT GGAAGAAGAAACCAACGATGAGGACATAACAGATATTGAGAATAAAATCGCTCGAAGATTGGCAGATAGA AAACAGCGGCTTTCTCAGGCTAACAACAAGCGGGATACTAGCAGCGATGCCGATTACGAGAACGACGATG ATGCGACCGCTGCCGCTGGAATCGGGGGAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102042 representing NP_604433
Red=Cloning sites Green=Tags MAGLLSTFDTFSSRRSESINKSGGGAIIPGQRSTVSVFTLGPSVTDDADKLLIATTFLAHSLDTDKQHSQ RGGFLVSLLAMAYSSPELYLTTNGVNADVKYVIYNIERDPKRTKTDGFIVKTRDMEYERTTEWLFGPMIN KNPLFQGQRENADLEALLQTYGYPACLGAIIVQVWIVLVKAITSSAGLRKGFFNRLEAFRQDGTVKSALV FTGDTVEGIGAVMRSQQSLVSLMVETLVTMNTSRSDLTTLEKNIQIVGNYIRDAGLASFMNTIKYGVETK MAALTLSNLRPDINKLRSLVDIYLSKGARAPFICILRDPVHGDFAPGNYPALWSYAMGVAVVQNKAMQQY VTGRTYLDMEMFLLGQAVAKDADSKISSALEEELGVTDTAKERLRHHLTNLSGGDGAYHKPTGGGAIEVA IDHTDITFGVEDTADRDNKNWTNDSNERWMNHSISNHTITIRGAEELEEETNDEDITDIENKIARRLADR KQRLSQANNKRDTSSDADYENDDDATAAAGIGGI TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_003461 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_003461.1, NP_604433 |
| RefSeq ORF | 1572 bp |
| Locus ID | 935258 |
| MW | 57.6 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102043 | Myc-DDK-tagged ORF clone of viral ORF for C' protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604434 |
CNY 3,800.00 |
|
| VC102044 | Myc-DDK-tagged ORF clone of viral ORF for phosphoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604435 |
CNY 6,840.00 |
|
| VC102045 | Myc-DDK-tagged ORF clone of viral ORF for C protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604436 |
CNY 3,600.00 |
|
| VC102046 | Myc-DDK-tagged ORF clone of viral ORF for Y1 protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604437 |
CNY 3,800.00 |
|
| VC102047 | Myc-DDK-tagged ORF clone of viral ORF for putative Y2 protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604438 |
CNY 3,800.00 |
|
| VC102048 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604439 |
CNY 4,180.00 |
|
| VC102049 | Myc-DDK-tagged ORF clone of viral ORF for F glycoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604440 |
CNY 4,136.00 |
|
| VC102050 | Myc-DDK-tagged ORF clone of viral ORF for HN glycoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604441 |
CNY 6,940.00 |
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