hypothetical protein (NC_001458) Virus Tagged ORF Clone

CAT#: VC101987

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV63gp3 [Human papillomavirus type 63], codon optimized for human cell expression, NP_040898



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CNY 7,410.00


货期*
5周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101987 represents NCBI reference of NP_040898 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGATAGGGGCACCAATAACGACGACTGGTACATTGTGGACGAAGCCGAGTGTCGCGACGATGATG
AGTCCGAACTGGAGGATCTGGAAGACACCTATAATTCTCTCTTTAACAGATCTGAGTCCGACATCTCCGA
CTTGTTGGATGACACGCAGCAATCCCAGGGTAATAGCTTGGAGCTCTTCCATTTGCAAGAACACTTGCAG
AACGAACAGGACCTCAATACACTTAAGCGGAAATACCTTAACAGCCCACCTCAGGCATCCGCTACAGAGA
CTGCTTGTAACTCCTTGTCCCCACGCCTCGAGAGTATTACCATTAGTCAGCGGGAAAAGAAGGCACGAAA
GCAGCTGTTCACCCAAAATGACTCTGGGATTGAGCTGAGCTTGTGTCAGGATGAAGTTGACAACATTAAC
GAGGCTTTGCAGGAACAGGTCGATATTGTCCAGTCACTTGGAGGCGGCGTCAGGGACTGCATTGGCGTAG
ACATCTTGAAGTGCTCTAATACTCGCTCTGCATTGCTGGCAAAATTTAAGGACACCGTGGGCGTGTCTTT
CACCGACCTCACACGCGCCTATAAGAACAATAAAACTTGTTGTAGCTATTGGGTCATCGCCGTGTGGGGC
GTTACCTCCACTTCAGTTGACGTGGTGAAAACAGTCTTTCAAGTTCAATGCAACTACATGCACGTGGAAC
ACTGTCTTACAGAGAAGAACAAGTTTCTGATCGTGCTGGCAGGTTTCAAAGCCCAGAAGAGCCGGGAGAC
CGTGTTGAATCTGGTTACCAGTTCTCTGAACGTCCAAAGCAACTATATTATGGCTGAACCCCCTAAGAAT
AGGAGCATGGCTGCAGCCCTTTATTGGTACCGCAGATCCATGTCTCCCGCCGTCTATACGTGGGGAGAGA
TGCCAGATTGGATGGCCCAGCAGACATTGCTGAATCACCAACTTGCCTCAGAGAAACACTTCGAGCTCAG
CCAGATGGTTCAGTGGGCGTACGACAACGGCTACACCGACGAGTCTGATATTGCATATTATTATGCGATC
CTCGCTGAGGAGGATGAGAATGCCAAAGCCTTTCTCGCTTCCAATGCCCAGGCCAAATACGTCAAAGACT
GCGCTAGGATGGTCTCTCACTACAAACGAGCCGAGATGTCCAGCATGTCCATGAGTGCATGGATTTATAA
AAGACTGGAAGAAGTTGAGAATGGCGGCGACTGGAAGCATATCGTTAAATTCCTGAGGTTCCAGGAGGTG
GAGTTTATCAGTTTTATGATTGCTTTTAAGGAACTGTTGAGTGGGAAACCAAAGAAGAACTGTTTGGTGA
TCTACGGACCGCCCAATACCGGTAAGTCAATGTTTTGTATGAGCCTCCTCCGCGTGTTGAAGGGCAAAGT
CATCTCATACGTGAATAGCAAATCCCAGTTTTGGCTGCAGCCCCTCGCGAGCACAAAAATTGCTCTGCTG
GACGATGCCACAAAGCCCGCCTGGGACTATATAGATCTCTTTCTCCGGAATGCCCTGGATGGGAACCCCA
TCTGCGTCGACCTTAAGCACAAAGCCCCTCAGCAGATTAAGTGTCCTCCCCTGATGATCACTTCCAACAT
CAACGTCAAAGCCGACGTGTGTTGGATGTATCTGCACAGCAGAATTACGTGTTTCGAGTTTAAACAGCCT
TTCCCTTTCGACGAAAATGGGCAGCCAGCATTTAGCCTGACCGATATTAACTGGAAAAGCTTCTTCGAGA
GGTTTTGGTCTCAACTCGATCTTAGTGACCAGGAGGATGAGGAGTCAGACGGGAAACCTCAACAGCCGCT
GAGGCTGGCCACCAGAGCAGCCAGCAATAGCATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101987 representing NP_040898
Red=Cloning sites Green=Tags

MTDRGTNNDDWYIVDEAECRDDDESELEDLEDTYNSLFNRSESDISDLLDDTQQSQGNSLELFHLQEHLQ
NEQDLNTLKRKYLNSPPQASATETACNSLSPRLESITISQREKKARKQLFTQNDSGIELSLCQDEVDNIN
EALQEQVDIVQSLGGGVRDCIGVDILKCSNTRSALLAKFKDTVGVSFTDLTRAYKNNKTCCSYWVIAVWG
VTSTSVDVVKTVFQVQCNYMHVEHCLTEKNKFLIVLAGFKAQKSRETVLNLVTSSLNVQSNYIMAEPPKN
RSMAAALYWYRRSMSPAVYTWGEMPDWMAQQTLLNHQLASEKHFELSQMVQWAYDNGYTDESDIAYYYAI
LAEEDENAKAFLASNAQAKYVKDCARMVSHYKRAEMSSMSMSAWIYKRLEEVENGGDWKHIVKFLRFQEV
EFISFMIAFKELLSGKPKKNCLVIYGPPNTGKSMFCMSLLRVLKGKVISYVNSKSQFWLQPLASTKIALL
DDATKPAWDYIDLFLRNALDGNPICVDLKHKAPQQIKCPPLMITSNINVKADVCWMYLHSRITCFEFKQP
FPFDENGQPAFSLTDINWKSFFERFWSQLDLSDQEDEESDGKPQQPLRLATRAASNSI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001458
ORF Size 1854 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001458.1, NP_040898
RefSeq ORF 1854 bp
Locus ID 1494574
MW 70.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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