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hypothetical protein (NC_001457) Virus Tagged ORF Clone
CAT＃: VC101928
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp4 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040892
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CNY 4,750.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101928 represents NCBI reference of NP_040892 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCCTTGGTGGCCCGATTTGATGCTCTGCAAGAAGCCATCCTCACTCACATTGAGTCCCAAGAGA GTACACTGGAGAGTCAGATCCAGTACTGGGAAAATATTCGCAAGGAGAACGCTATTATGCATTATGCTAG GAAGCAAGGCCTCACTAAATTGGGACTTCAGCCCTTGCCCACACTGGCGGTCACTGAGTACAATGCCAAG CAAGCAATTCAAATCCACCTCACGCTGCAGAGTCTCCTTAAGTCTCCCTTCGCCTCCGAACGCTGGACCT TGACAGACGTGAGCGCTGAGCTGATTAATACTTCACCACAGAACTGCCTGAAAAAAGGAGGGTACGACGT CGCCGTGTGGTTTGATAATGACCGGCAGAACGCAATGTTGTATACTAACTGGGATTTTCTCTATTATCAG GACATGAATGAACAATGGCACAAAGTGAAGGGCGAGGTGGATTACGACGGTCTTTACTTTACAGATCATA CCGGCGAGCGCGCCTACTTCACTCTCTTCAGCTCTGACGCACAGAGATTCTCCAGAACAGGGCTGTGGAC GGTTCATTTCAAAACTCAAGTAATCTCAAGCCCGATTGTGAGTAGCACCTACTCTAGCTCTTTTGACACA GAGGAGCAACAGCTTCCTGGCCCCTCAACCTCCTACAGCGAGGTGACCGAGCAAGCGTCCCCCACTCGAA GACGAAAGCCAAGGAAATCCGACGCAACTTCCACAACAAGTCCAGAAACAGAAGGAGTCCGCTTGAGACG GCGGCGACGGGAGGGAAAATCAGGCCCAGGCAGTGGCGAAACGCCTAGAAAGCGGAGGAGAGGAGGCGGT AGGGGCGGAGGGGAAACAGAGCTTGGATCCGCACCTTCCCCCGCCGAGGTGGGCAGTAGGCATCGACAGG TTGAGAGACAGGGGCTCAGCAGGCTGGGCCTTCTGCAGGCAGAGGCCAGGGACCCACCAATGATCTTGCT CAAGGGGACCGCCAATTCACTCAAGTGCTGGCGGTACCGGAAGGTGAACAGCAATTGCTGTAACTTCCTC TTCATGTCTACTGTGTGGAATTGGGTTGGAGACTGCAGTCACAATCACAGCCGCATGTTGATCGCATTCG ACTCCACAGATCAGCGGGATGCTTTTGTCAAGCATAATCTTTTCCCCAAGCTGTGTACGTATACCTACGG GTCTCTCAACAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101928 representing NP_040892
Red=Cloning sites Green=Tags MESLVARFDALQEAILTHIESQESTLESQIQYWENIRKENAIMHYARKQGLTKLGLQPLPTLAVTEYNAK QAIQIHLTLQSLLKSPFASERWTLTDVSAELINTSPQNCLKKGGYDVAVWFDNDRQNAMLYTNWDFLYYQ DMNEQWHKVKGEVDYDGLYFTDHTGERAYFTLFSSDAQRFSRTGLWTVHFKTQVISSPIVSSTYSSSFDT EEQQLPGPSTSYSEVTEQASPTRRRKPRKSDATSTTSPETEGVRLRRRRREGKSGPGSGETPRKRRRGGG RGGGETELGSAPSPAEVGSRHRQVERQGLSRLGLLQAEARDPPMILLKGTANSLKCWRYRKVNSNCCNFL FMSTVWNWVGDCSHNHSRMLIAFDSTDQRDAFVKHNLFPKLCTYTYGSLNSL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001457 |
| ORF Size | 1206 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001457.1, NP_040892 |
| RefSeq ORF | 1206 bp |
| Locus ID | 1489449 |
| MW | 45.8 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101925 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp1 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040889 |
CNY 3,800.00 |
|
| VC101926 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp2 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040890 |
CNY 3,800.00 |
|
| VC101927 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp3 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040891 |
CNY 7,220.00 |
|
| VC101929 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp5 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040893 |
CNY 3,800.00 |
|
| VC101930 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp6 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040894 |
CNY 6,370.00 |
|
| VC101931 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV4gp7 [Human papillomavirus type 4], codon optimized for human cell expression, NP_040895 |
CNY 6,270.00 |
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