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E1 (NC_014956) Virus Tagged ORF Clone
CAT＃: VC101885
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169293
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CNY 7,220.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101885 represents NCBI reference of YP_004169293 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACAAGAAGGGCATCAAGAGCAGCAAGCTGTCCAACAAATGCAAAGAGTGGTTCATCATTGAGG AAGCCGACTGCGACGAGCTGACAGAGATGGATAAAATCTTCGAAGAATCCGACGGCAGCATCGTGAGTAA CCTGCTGGATGACAGTGATGAGGTCGACCAAGGCAATTCACTTGAGCTGCTTAATGAGCAGCTCCTGCAG GAGAGCAATCTGCAACTGGCTGACTTGAAGAGAAAATATCTCAGCCCCGAGAAGGATTCTGTGTTGGCCC TGAGTCCTCAGCTCGAGAGCGTAAGCATTAGCCCTCACCCAAAGAACTCACGACGCCGCCTGTTTGATGA CTCAGGAATAGAGAACGAAGCCGAAGACAGTATCGGTGTCACCCAGGTCGAGAGCGTGTCCCCAGACTGC CAGACAGTGGATGATGGAGCCAGGGTCTGTAAAGAGCTGCTTCAGTCTAAGAATCGGAGAGCAATGTACC TGGCTAAGTTCAAGGAATCATTTGGGGTGTCTTTCACCGAGCTGACGCGGGTGTTCAGGAGTGATAAGAC GTGTTGCAACAGCTGGGTGATCTGTGTCCTCGCGGTGAACGAAGAACTCGTGGAGTCTAGTAAGACGCTC CTCAAACTTCATTGCGAGTTCTGTCAGATTCTCGTGCCGACACTCGGGCCAGTGGTTACCGCGCTGTATC TCTGCGAGTTTAAATGTTCCAAAAACCGCGAAACGGTGACAAAGTTGTTCTGCCAGCTCCTCAATGTTCA AGAAATGCAACTGATGTGCAACCCTCCCAAGCATAAGAGCGTGGTCGTCGCGTTGTTCTTTTTTAAGCAG AGCATGAGTAGTATTAGTTTCAAGTTCGGCGCGTTCCCAGAGTGGCTCGCCAAACAGACCGTGGTGTCCT TCCAACAGGAGGCGGAAACGTTCCAGCTGAGCAGAATGGTGCAGTGGGCTTACGATCACAATTACGTGGA AGAATCTGTCATCGCGTACCACTACGCCTGCATAGCCAACGAAGATTCCAACGCCGCAGCCTGGCTCAAT TGTAATAATCAGCTGAAGTATGTCCATGATTGTTCCAGAATGGTGAGGTACTACATAAGACAAGAAATGA GAGAAATGACTATCTCAGAGTGGATATACAAATGCTGTGACAAAGTGAAAGACACCAGCGACGAATGGAA GGTGATAGCTAAACTCCTGCGGTACCAGGAGGTGAATATGGTGGCGTTCCTCTGCGCTCTGAGGCTTATG TTCAAGAAAGTGCCAAAGAAGAATACCATAGTTTTGAGCGGTAAACCTAATTCCGGTAAAAGCTATTTTT CATACAGTCTCATGAGTTTTCTGGAAGGGAAAGTCGTGAGTCATATGAACAGTAAGTCCCACTTTTGGCT TCAACCCCTGACCGATTCAAAGTTCGGTCTCATCGATGACGCTACTGACGCATGCTGGCAGTTCATGGAT GTTTATATGAGGAGTGCACTGGACGGAAATGACATTTCTGTCGATTGCAAGCACAAGAACGCTATGCAAG TGAAATTGCCCAGTCTCTTCATTACCACTAACGTGCCTGTGGACAAAGAAATACAGTACAGTTACCTTCA TTCAAGGCTCCAGGTGTTTTATTTCAACCGCGAGATGCCGTTGGACTCCGCCGGTAATCCTGTTTACGAG ATTAACGACAAGACCTGGGCATCATTTTTCATAAAGCTGGAAAAACAGCTGGATCTGACCCGGCCGGAGG ACGGGAAACCCGACAGAGCCTTTCGCTGTAGCGCTGGCGAACCTGCTGGAATTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101885 representing YP_004169293
Red=Cloning sites Green=Tags MADKKGIKSSKLSNKCKEWFIIEEADCDELTEMDKIFEESDGSIVSNLLDDSDEVDQGNSLELLNEQLLQ ESNLQLADLKRKYLSPEKDSVLALSPQLESVSISPHPKNSRRRLFDDSGIENEAEDSIGVTQVESVSPDC QTVDDGARVCKELLQSKNRRAMYLAKFKESFGVSFTELTRVFRSDKTCCNSWVICVLAVNEELVESSKTL LKLHCEFCQILVPTLGPVVTALYLCEFKCSKNRETVTKLFCQLLNVQEMQLMCNPPKHKSVVVALFFFKQ SMSSISFKFGAFPEWLAKQTVVSFQQEAETFQLSRMVQWAYDHNYVEESVIAYHYACIANEDSNAAAWLN CNNQLKYVHDCSRMVRYYIRQEMREMTISEWIYKCCDKVKDTSDEWKVIAKLLRYQEVNMVAFLCALRLM FKKVPKKNTIVLSGKPNSGKSYFSYSLMSFLEGKVVSHMNSKSHFWLQPLTDSKFGLIDDATDACWQFMD VYMRSALDGNDISVDCKHKNAMQVKLPSLFITTNVPVDKEIQYSYLHSRLQVFYFNREMPLDSAGNPVYE INDKTWASFFIKLEKQLDLTRPEDGKPDRAFRCSAGEPAGII TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_014956 |
| ORF Size | 1806 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_014956.1, YP_004169293 |
| RefSeq ORF | 1806 bp |
| Locus ID | 10146756 |
| MW | 69.0 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101886 | Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169294 |
CNY 5,130.00 |
|
| VC101887 | Myc-DDK-tagged ORF clone of viral ORF for E4 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169295 |
CNY 3,800.00 |
|
| VC101888 | Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169291 |
CNY 3,800.00 |
|
| VC101889 | Myc-DDK-tagged ORF clone of viral ORF for E7 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169292 |
CNY 3,800.00 |
|
| VC101890 | Myc-DDK-tagged ORF clone of viral ORF for L1 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169297 |
CNY 6,270.00 |
|
| VC101891 | Myc-DDK-tagged ORF clone of viral ORF for L2 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169296 |
CNY 6,270.00 |
