E2 (NC_012485) Virus Tagged ORF Clone

CAT#: VC101778

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756541



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CNY 4,940.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101778 represents NCBI reference of YP_002756541 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAAACGTTGACCGAACGGTTCGACGCCCTGGAAGAGAACCTCCTCCAATTGTATGAGGCCGGAT
CCGATAAGATTGACGATCAGATTCTGTACTGGGACATTATTCGCCAGGAAAACGTTCTTTTCTTCTACGC
TAGGAAGAAGGGCCTTCAGAGGATTTCCCTGCAGCCAGTTCCCAGCCTGACCGTCTCCGAACATAAGGCT
AAACAAGCTATCATGATGAGTATACAGTTGACTAGCCTGAAGAACAGCGCTTTCGGTAACGAACCTTGGA
CTCTGCAGGACACTTCCTTTGAGCTCTATAATGCGCCACCGCAGAATACATTTAAAAAACAGGCTTTTAC
TGTGGATGTTTTTTATGACAACGACGAGGACAATTATTACCCGTATACTGCCTATAAATTTATTTATTAC
CAGAATGGGGATAACATCTGGCATAAAGTGGAGAGCGACGTGGACTACGAGGGTCTTTTCTACATAACTC
ATGACTATGAAAAAGTGTATTACGTGACATTCGACCAGGACGCTCGGCGGTTTTCCAGAACCGGGCACTG
GACTGTTAAGTACAAGAATCGAACCATCTCATCCACCTCAGTGACAAGCACCTCTGGCACCCCACCTGAA
CATCCCAGTTCCAGTCGCTCTGGTGCCAACACATTGGCTAGACAGACTGAGATAGCCCCACAGGAACGCA
GACGGCCCCGATCCAGTTCAGAAAGCCCTCAGAGACATACTCGCCCTCGAACCTCCCAGTCCGACGGGGA
TACTAGTGCGTCAACCCCCCGGCGGAGGCTCAGACGAAGAAGAAGAGAGAGGGAGCCAGCCGCACAGCGC
AGAGAACAGAGAAAGACTAATACCACTCGGTCCTCCTACCCAAGCCCGGAGGCAGTCGGACGCAGGCATA
GGGTTGTGGAGGGTAAGGGACTGTCTCGGCTCGGAAGGTTGCAGGAGGAAGCACGAGGTCCACCTCTGAT
CTGTCTGAAGGGACCTGCAAACACCCTGAAGTGTTGGAGGTATCGGTGTAAACTGAAATATCGCGGCATG
TTCTACAGGATCAGTACCGGATTCAGCTGGGTGGCTGAAGGTCCAGCTCGCCTGGGCGACCAACGAATGT
TGATCGCTTTTCACAACTCTGCTCAGCGCCAAGCCTTCCTCAAGATCGCCGCGTTTCCTAAAGGGACCCA
ATTCGCGCTCGGAAATCTGGATTCACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101778 representing YP_002756541
Red=Cloning sites Green=Tags

MMETLTERFDALEENLLQLYEAGSDKIDDQILYWDIIRQENVLFFYARKKGLQRISLQPVPSLTVSEHKA
KQAIMMSIQLTSLKNSAFGNEPWTLQDTSFELYNAPPQNTFKKQAFTVDVFYDNDEDNYYPYTAYKFIYY
QNGDNIWHKVESDVDYEGLFYITHDYEKVYYVTFDQDARRFSRTGHWTVKYKNRTISSTSVTSTSGTPPE
HPSSSRSGANTLARQTEIAPQERRRPRSSSESPQRHTRPRTSQSDGDTSASTPRRRLRRRRREREPAAQR
REQRKTNTTRSSYPSPEAVGRRHRVVEGKGLSRLGRLQEEARGPPLICLKGPANTLKCWRYRCKLKYRGM
FYRISTGFSWVAEGPARLGDQRMLIAFHNSAQRQAFLKIAAFPKGTQFALGNLDSL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_012485
ORF Size 1218 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_012485.1, YP_002756541
RefSeq ORF 1218 bp
Locus ID 7701330
MW 47.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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